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| 激光辐照花生种子的细胞学效应 | |
| 引用本文： | 欧琳,陈荣,庄伟建,肖华山.激光辐照花生种子的细胞学效应[J].激光生物学报,2006,15(2):140-143. |
| 作者姓名： | 欧琳 陈荣 庄伟建 肖华山 |
| 作者单位： | 1. 福建师范大学物理与光电信息学院,福建,福州,350007 2. 福建农林大学作物科学院,福建,福州,350007 3. 福建师范大学生物工程学院,福建,福州,350007 |
| 基金项目： | 福建省自然科学基金项目(F9810011) |
| 摘 要： | 本试验考察了用Ar (488 nm)激光辐照对花生种子胚轴细胞形态结构以及根尖细胞染色体的影响。研究结果表明:在辐照功率密度为5.12 W/cm2,时间为10 s、25 s、55 s情况下,胚轴细胞均有不同程度的损伤;且均能诱发根尖细胞染色体畸变。处理时间延长,细胞破坏加剧,主要表现在细胞中膜系统、脂体、蛋白体和细胞核,出现了异形、合并或结构混乱等现象。染色体畸变类型也随之增加。各激光处理剂量均对花生种子萌发产生抑制作用。 |
| 关 键 词： | 激光辐照 花生种子 染色体畸变 超微结构 |
| 文章编号： | 1007-7146（2006）02-0140-04 |
| 收稿时间： | 2005-08-01 |
| 修稿时间： | 2005-10-10 |
Cytological Effect of Laser-irradiation on Peanut Seeds |
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| OU Lin,CHEN Rong,ZHUANG Wei-jian,XIAO Hua-shan.Cytological Effect of Laser-irradiation on Peanut Seeds[J].ACTA Laser Biology Sinica,2006,15(2):140-143. | |
| Authors: | OU Lin CHEN Rong ZHUANG Wei-jian XIAO Hua-shan |
| Institution: | 1. Fujian Normal University a. School of Physics and Optoelectronic Technology; b. Biological Engineering Institute, Fuzhou 350007, Fujian, China; 2. Crop Science Institute, Fujian Agriculture and Forest University, Fuzhou 350007, Fujian, China |
| Abstract: | In this present paper,dry seeds of peanut of cytological effects have been studied by Ar~ laser-irradiation at 488 nm.With laser 5.12 W/cm~2 power density,there was difference in the plumule cell and cell nucleus,and the chromosomal aberration of peanut root tip cell was induced and observed.With irradiating time increased,the cells were destroyed seriouly,such as on the membrane system,fatbody,inhibition proteinbody and cell nucleus appeared irregular,and the types of chromosomal aberration also increased.According to the seeds sprouting and growing,the dose of 60 s treatment was a suitable dose. |
| Keywords: | laser irradiation peanut seeds chromosomal aberration ultrastructure |
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