| 家蚕Bms3a基因真核表达载体的构建及其在家蚕细胞和蚕体中的表达 |
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| 引用本文： | 尤征英,周武松,徐家萍,杨莹,凌琳,王文兵.家蚕Bms3a基因真核表达载体的构建及其在家蚕细胞和蚕体中的表达[J].激光生物学报,2011,20(2):212-218. |
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| 作者姓名： | 尤征英 周武松 徐家萍 杨莹 凌琳 王文兵 |
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| 作者单位： | 1. 安徽农业大学生命科学学院,安徽,合肥,230036
2. 江苏大学生命科学研究院,江苏,镇江,212013 |
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| 基金项目： | 国家自然科学基金项目，安徽省科技攻关项目 |
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| 摘 要： | Bms3a基因可能在家蚕Bombyx mori抗病或细胞凋亡中有一定的作用。将融合有绿色荧光蛋白的Bins3a基因克隆到杆状病毒转移载体pFastBac1中获得了pFastBac-IE1-Bms3a-EGFP真核表达载体,利用杆状病毒(Bac-to-Bac)表达系统筛选重组杆状病毒,以重组病毒感染家蚕BmN细胞和五龄幼虫,分别在感染24h和48h检测到有绿色荧光蛋白的表达,Western blot证明表达的融合蛋白在相对分子量约57kD处出现特异条带,与预计的蛋白理论值相符。结果表明BmS3A-EGFP融合蛋白在家蚕细胞BmN及幼虫体中得到高效表达。研究结果为进一步研究BmS3A蛋白的功能奠定了基础。
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| 关 键 词： | 家蚕 Bms3a基因 真核表达 杆状病毒表达系统 |
Construction of Bms3a Eukaryotic Expression Vector and Its Expression in BmN Cells and Silkworm, Bombyx mori |
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| YOU Zheng-ying,ZHOU Wu-song,XU Jia-ping,YANG Ying,LING Lin,WANG Wen-bing.Construction of Bms3a Eukaryotic Expression Vector and Its Expression in BmN Cells and Silkworm, Bombyx mori[J].ACTA Laser Biology Sinica,2011,20(2):212-218. |
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| Authors: | YOU Zheng-ying ZHOU Wu-song XU Jia-ping YANG Ying LING Lin WANG Wen-bing |
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| Institution: | YOU Zheng-ying~1,ZHOU Wu-song~1,XU Jia-ping~(1*),YANG Ying~1,LING Lin~1,WANG Wen-bing~2 (1.School of Life Sciences,Anhui Agricultural Universily,Hefei 230036,Anhui,China,2.Institute of Life Sciences,Jiangsu University,Zhenjiang 212013,Jiangsu,China) |
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| Abstract: | Bms3a gene may be involved in silkworm resistance and anti-apoptosis.The fragment of Bms3a gene with EGFP has been inserted into a transfer vec-tor pFastBacl and the recombination baculovims was obtained.The green fluorescence can be observed in the BmN cells and the 5th instar larvae after 24 h and 48 h by infection of the recombinant virus,respectively.Western blot showed specific reaction band with a relative molecular mass of about 57 kD,which was consistent with predicted size of the target protein.The... |
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| Keywords: | Bombyx mori Bms3a gene eukaryotic express baculovirus system |
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