| N+离子注入诱变选育纤维素酶高产菌 |
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| 引用本文： | 陈阿娜,汤斌,刘标.N+离子注入诱变选育纤维素酶高产菌[J].激光生物学报,2011,20(2):171-174. |
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| 作者姓名： | 陈阿娜 汤斌 刘标 |
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| 作者单位： | 安徽工程大学生物与化学工程学院,安徽,芜湖,241000 |
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| 基金项目： | 安徽省高校自然科学基金重点资助项目，安徽工程科技学院青年科研基金项目 |
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| 摘 要： | 纤维素酶产生菌Rhizopus sp.TCl经过N+离子注入、紫外线、5-氟尿嘧啶(5-Fu)等诱变剂诱变,选育得到突变株Rhizopus sp.TCl653,发酵终点时滤纸酶活力(FPA)和羧甲基纤维素酶活力(CMC)分别为22.6 IU/g、224.7 IU/g干物质,较出发菌株分别提高了276.7%和214.3%...
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| 关 键 词： | 纤维素酶 N+离子注入 传代培养 发酵周期 |
Screening of High-yield Cellulase-producing Strains by N+ Implantation Mutation |
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| CHENA A-na,TANG Bin,LIU Biao.Screening of High-yield Cellulase-producing Strains by N+ Implantation Mutation[J].ACTA Laser Biology Sinica,2011,20(2):171-174. |
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| Authors: | CHENA A-na TANG Bin LIU Biao |
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| Institution: | CHEN A-na,TANG Bin,LIU Biao (Department of Biochemical Engineering,Anhui Polytechnic University,Wuhu 241000,Anhui,China) |
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| Abstract: | Cellulase-producing strain Rhizopus sp.TC1 was subjected to mutation involving treatment of N~+implantation, UV followed by 5-Fu;successive mutant Rhizopus sp.TC1653 showed enhanced cellulase production.The FPA and CMC case of mutant TC1653 was 22.6 and 224.7 IU per gram of dry medium respectively at the end of fermentation, which was increased by 3.77 folds and 3.14 folds respectively compared to that of TCI.Ten times subculturing indicated that the mutant TC1653 had good genetic stability and character of... |
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| Keywords: | cellulase N~ implantation subculture fermentation period |
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