| 离轴抛物镜扫描中继系统提升双光子显微成像视场 |
| |
| 引用本文： | 姚靖,吴婷,叶世蔚,高玉峰,郑炜,秦水介.离轴抛物镜扫描中继系统提升双光子显微成像视场[J].激光生物学报,2020,29(3):217-224. |
| |
| 作者姓名： | 姚靖 吴婷 叶世蔚 高玉峰 郑炜 秦水介 |
| |
| 作者单位： | 贵州大学 物理学院, 贵阳 550025;贵州大学 贵州省光电子技术与应用重点实验室, 贵阳 550025;中国科学院深圳先进技术研究院生物医学光学与分子影像研究室,深圳518055;中国科学院深圳先进技术研究院生物医学光学与分子影像研究室,深圳518055;贵州大学 物理学院, 贵阳 550025;贵州大学 贵州省光电子技术与应用重点实验室, 贵阳 550025 |
| |
| 基金项目： | 深圳市基础研究项目;国家自然科学基金 |
| |
| 摘 要： | 本文提出利用离轴抛物镜共焦中继系统来改善双光子成像视场边缘像质变差的问题,进而提升双光子成像视场。不同于传统的双胶合透镜扫描中继系统,离轴抛物镜共焦扫描中继系统由一对离轴抛物镜构成,扫描振镜位于抛物面镜焦点处。仿真和试验结果均显示,该系统能有效优化视场边缘的像散、场曲和畸变情况,提高成像质量。利用该扫描系统,我们实现了视场为2.4 mm×2.4 mm,横向分辨率为1μm的大视场双光子显微成像,能清晰分辨小鼠大脑切片中的神经轴突结构。
|
| 关 键 词： | 离轴抛物面反射镜 双光子成像 共焦扫描中继 像差 大视场 |
Off-axis Parabolic Mirror Afocal Scanning System Extends the Imaging Area of Two-photon Microscopy |
| |
| YAO Jing,WU Ting,YE Shiwei,GAO Yufeng,ZHENG Wei,QIN Shuijie.Off-axis Parabolic Mirror Afocal Scanning System Extends the Imaging Area of Two-photon Microscopy[J].ACTA Laser Biology Sinica,2020,29(3):217-224. |
| |
| Authors: | YAO Jing WU Ting YE Shiwei GAO Yufeng ZHENG Wei QIN Shuijie |
| |
| Institution: | (Guizhou University Department of Physics,Guiyang 550025,China;Guizhou University,Key Laboratory of Optoelectronic Technology and Application of Guizhou,Guiyang 550025,China;Research Laboratory for Biomedical Optics and Molecular Imaging,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China) |
| |
| Abstract: | We propose an off-axis parabolic mirror(OAPM)afocal scanning system which can improve the image quality in the marginal area of two-photon microscopy.Compared with the traditional double lenses afocal scanning system,the OAPM afocal scanning system consists of a pair of parabolic mirrors,and a galvo scanner is located at one of the foci of the parabolic mirror.Both the simulation and experiment results show that the OAPM afocal scanning system can significantly reduce the astigmatism,the field curvature and the distortion introduced on the edge of field of view.Furthermore,adopting the proposed afocal scanning system,we build a two-photon microscope with a field of view of 2.4 mm×2.4 mm and a lateral resolution of 1μm.Using this system,the neuron axons in the mouse brain slice can be clearly resolved. |
| |
| Keywords: | off-axis parabolic mirror two-photon microscopy afocal scanning relay aberration large field-of-view |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
