| 醋酸铅对体外培养大鼠肾小管上皮细胞的毒性损伤 |
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| 引用本文： | 洪玮,付欣,周问渠,邹东霆,李冰.醋酸铅对体外培养大鼠肾小管上皮细胞的毒性损伤[J].激光生物学报,2011,20(3):315-318. |
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| 作者姓名： | 洪玮 付欣 周问渠 邹东霆 李冰 |
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| 作者单位： | 广州医学院实验医学研究中心,广东广州,510182 |
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| 基金项目： | 广州医学院青年科学研究项目 |
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| 摘 要： | 探讨醋酸铅对体外培养的大鼠肾小管上皮细胞系HBZY-1的细胞毒性效应.用不同浓度的醋酸铅(30、40、50 μmol/L)作用HBZY-1细胞24 h、48 h和72 h,采用CellTiter-Glo(R)萤光细胞活性检测盒测定细胞存活情况;用流式细胞仪检测细胞凋亡率.结果显示经30、40、50μmol/L醋酸铅处理...
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| 关 键 词： | 醋酸铅 肾小管上皮细胞HBZY-1 细胞存活 细胞凋亡 |
The Toxic Effect of Lead Acetate on Rat Renal Tubular Epithelial Cell HBZY-1 |
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| HONG Wei,FU Xin,ZHOU Wen-qu,ZOU Dong-ting,LI bing.The Toxic Effect of Lead Acetate on Rat Renal Tubular Epithelial Cell HBZY-1[J].ACTA Laser Biology Sinica,2011,20(3):315-318. |
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| Authors: | HONG Wei FU Xin ZHOU Wen-qu ZOU Dong-ting LI bing |
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| Institution: | HONG Wei,FU Xin,ZHOU Wen-qu,ZOU Dong-ting,LI bing~* (Experimental Medical Research Center,Guangzhou Medical University,Guangzhou 510182,Guangdong,China) |
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| Abstract: | To explore the toxic effect of lead acetate on rat ren 1 tubular epithelial cell HBZY-1. HBZY-1 cells were treated with different concentrations of lead acetate（30,40 and 50 p, mol/L） for 24 h,48 h and 72 h. After 48 hours,using CellTiter-GloTM Luminescent Cell Viability Assay determined cell activity; the apoptosis rate was measured by flow eytometry. The results shows that cell activity decreased significantly（ P 〈 0.05 ） by 30,40 and 50 μmol/L lead acetate treatment HBZY-I cells after 72 h; Flow cytometry results showed that the cell apoptosis rate was higher in the treatment group than in the control group. Lead acetate can remarkably inhibit rat renal tubular epithelial HBZY-I cell growth, promote apoptosis and affect the kidney. |
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| Keywords: | lead acetate kidney tubular epithelial cells cell activity apoptosis |
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