| CXXC5多克隆抗体的制备及表达研究 |
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| 引用本文： | 王西君,廖鹏,王跃群,邓云,莫小阳,袁婺洲,万永奇,吴秀山,李永青.CXXC5多克隆抗体的制备及表达研究[J].激光生物学报,2011,20(3):334-337,328. |
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| 作者姓名： | 王西君 廖鹏 王跃群 邓云 莫小阳 袁婺洲 万永奇 吴秀山 李永青 |
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| 作者单位： | 湖南师范大学蛋白质化学及鱼类发育生物学教育部重点实验室,心脏发育研究中心,湖南长沙410081 |
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| 基金项目： | 国家自然科学基金项目，国家重点基础研究发展计划资助项目 |
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| 摘 要： | CXXC5基因是从人类胚胎心脏的cDNA文库中克隆出来的一个人类锌指基因,包含zf-CXXC5结构域,该基因编码322个氨基酸,在物种进化上高度保守.为了进一步研究该基因的功能,需要获得CXXC5蛋白并制备其抗体.通过PCR扩增方法扩增得到了CXXC5部分编码区序列,然后将其连接到PGEX4T-1上,转化到大肠杆菌BL...
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| 关 键 词： | CXXC5 融合蛋白 多克隆抗体 |
A Preparation of Polyclonal Antibody and Expression of Human CXXC5 Gene |
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| WANG Xi-jun,LIAO Peng,WANG Yue-qun,DENG Yun,MO Xiao-yang,YUAN Wu-zhou,WAN Yong-qi,WU Xiu-shan,LI Yong-qing.A Preparation of Polyclonal Antibody and Expression of Human CXXC5 Gene[J].ACTA Laser Biology Sinica,2011,20(3):334-337,328. |
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| Authors: | WANG Xi-jun LIAO Peng WANG Yue-qun DENG Yun MO Xiao-yang YUAN Wu-zhou WAN Yong-qi WU Xiu-shan LI Yong-qing |
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| Institution: | WANG Xi-jun,LIAO Peng,WANG Yue-qun,DENG Yun,MO Xiao-yang,YUAN Wu-zhou,WAN Yong-qi,WU Xiu-shan,LI Yong-qing~* (The Center for Heart Development,Key Lab of MOE for Development Biology and Protein Chemistry,Hunan Normal University,Changsha 410081,Hunan,China) |
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| Abstract: | CXXC5 was cloned from a human heart cDNA library and identified as human zf-CXXC-related zinc finger gene encoding a 322 amino acid protein.CXXC5 is an evolutionarily conserved protein.Obtianing the CXXC5 protein and it's antibody is essential for the further studies.A cDNA fragment of partial coding sequence of CXXC5 was amplified by PCR,then the PCR product was cloned into the expression vector pGEX-4T-1 and transformed into Escherichia coli BL21(DE3).The fusion protein of the recombinant expression plasm... |
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| Keywords: | CXXC5 |
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