三种果蝇精巢生殖干细胞半衰期的研究

果蝇精巢生殖干细胞(Germline stem cells,GSCs)的数量伴随衰老呈现递减趋势。本文以两种野生型果蝇品系Oregon、W1118以及Stat基因突变体的精巢为研究材料,采用免疫荧光染色技术,标记果蝇精巢Hub组织及生殖干细胞,研究了室温(25℃)条件下三种果蝇羽化后六个时间点(第1、15、30、45、60、75 d)精巢GSC的数量。结果表明,两种野生型Oregon、W1118与stat突变体果蝇羽化后第1 d的GSC平均数分别为8.55、8.60与8.15,根据曲线图得到,两种野生型果蝇GSCs半衰期均为60 d左右,而stat突变体果蝇约为30 d,两者存在显著差异,表明stat基因突变加速了果蝇精巢GSCs的衰老。

Study on the Half-life of Testis Germline Stem Cells in Three Lines of Drosophila

The number of germline stem cells(GSCs)in Drosophila testis decreases with aging.Using immuno-fluores-cent staining technique,the testis GSCs number of three lines of Oregon,W1118 and Stat mutant flies is studied in different stage(day 1,15,30,45,60 and day 75 after eclosion)at room temprature.The average GSCs number of the aboved flies in day 1 after eclosion is respectively 8.55,8.60 and 8.15,the half-life of the two wild-type lines(Oregon,W1118)and the Stat mutant line are respectively sixty days and thirty days based on the curve generated on the relationship of the GSCs number and the elapsed days.There is a big difference in the half-life of testis GSCs between the wildtype and Stat mutant flies.The result indicates that gene Stat mutation dramatically accelerate the aging of GSCs in Drosophila testis.