重离子及电子辐照沙打旺(Astragalus Adsurgens Pall)干种子的辐射生物学效应

本文采用80MeV/u12C6+和50keV的电子辐照沙打旺干种子,研究沙打旺干种子不同注量的辐照生物学效应,检测其生理生化指标。结果表明:无论是碳离子辐照还是电子辐照,发芽势都随注量的增加先升高后降低,发芽率都随注量增加逐渐降低。其次,碳离子辐照样品,其超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性变化随注量增加先升高后降低,即存在一个峰值,过氧化氢酶(CAT)在高于5×106ions/cm2注量的离子辐照中其活性基本不发生变化;不同注量的电子辐照,样品的CAT、SOD、GSH-Px活性都是先升高后降低。第三,无论是碳离子辐照还是电子辐照样品的谷胱甘肽(GSH)含量变化不显著,与注量的变化没有明显的相关性。总而言之,本工作发现碳离子及电子辐照样品,其多个生理生化指标均随注量的增加呈先升高后降低的趋势,但GSH变化对碳离子或电子辐照都不敏感。

Radiobiological Effects of 12C6+ Ion and Electron Beams with Different Fluence on Astragalus Adsurgens Pall Dry Seeds

The radiobiological effects with regard to physiological and biochemical characters of dry Astragalus adsurgens Pall seeds which were irradiated by 80MeV/u 12C6+ ion and 50 keV electron beams with different doses were studied in this paper. Experimental results show that the germination potential of the samples irradiated by both carbon ions and electrons increases first and then decreases with dose, while their germination fraction goes down gradually. In the samples irradiated by12C6+ ions, their activities of superoxide dismutase and glutathione peroxidase rise first and then decline, that means there maybe a peak in their change; their activity of catalase does not vary anymore when the irradiation fluence is more than 5×107ions/cm2. In the samples irradiated by electron with different doses, their activities of catalase, superoxide dismutase and glutathione peroxidase all go up first and then fall. Content of glutathione does not vary basicly with dose in whichever carbon ion irradiation......