| N+注入诱变选育菜粕硫苷降解菌的研究 |
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| 引用本文： | 景志远,李吕木,丁小玲,许发芝,刘海进.N+注入诱变选育菜粕硫苷降解菌的研究[J].激光生物学报,2011,20(4):468-473. |
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| 作者姓名： | 景志远 李吕木 丁小玲 许发芝 刘海进 |
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| 作者单位： | 1. 安徽农业大学动物科技学院,安徽合肥,230036
2. 安徽农业大学动物科技学院,安徽合肥230036;安徽省畜牧生物工程技术研究中心,安徽合肥230031 |
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| 基金项目： | 国家科技部科技人员服务企业行动项目(2009GJC30021); 安徽省农业科技成果转化项目(09150306006) |
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| 摘 要： | 为选育能够高效降解菜粕硫苷( Glucosinolates,Gls)的菌株,利用能降解硫苷的益生菌黑曲霉CICC-2238进行N+注入诱变.经过两次N+注入诱变,在诱变能量为20 keV,剂量为60 ×2.5×1013ions/cm2时,筛选到一株高效降解Gls的菌株H-109.与出发菌相比较,其Gls的降解率由28....
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| 关 键 词： | 菜粕 硫代葡萄糖苷 黑曲霉 N+离子注入 诱变育种 |
A Study on the Breeding by Ion Implantation and Cultivation of the Rapeseed Meal Glucosinolates Degrading Strain |
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| JING Zhiyuan,LI Lvmu,DING Xiaoling,XU Fazhi,LIU Haijin.A Study on the Breeding by Ion Implantation and Cultivation of the Rapeseed Meal Glucosinolates Degrading Strain[J].ACTA Laser Biology Sinica,2011,20(4):468-473. |
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| Authors: | JING Zhiyuan LI Lvmu DING Xiaoling XU Fazhi LIU Haijin |
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| Institution: | JING Zhiyuan~1,LI Lvmu~(1,2*),DING Xiaoling~(1,2),XU Fazhi~(1,LIU Haijin~1 (1.College of Animal Science and Technology,Anhui Agriculture University,Hefei 230036,Anhui,China,2.Anhui Animal Biological Engineering Technology Research Center,Hefei 230031,China) |
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| Abstract: | To obtain an efficient rapeseed meal glucosinolates degrading strain by N ~ ion implantation. Aspergillus ni- ger CICC-2238 was original probiotic, which was implanted by N ~ ion with dose of 60 ×2.5 × 1013 ions/cm2 under im- plantation energy for 2 times and a high-yield mutant strain H-109 was obtained. Compared with the original strain, the glucosinolate degradation rate of mutant strains was enhanced from 28.95% to 49.88%. The results showed that N ~ ion implantation mutation was a successful approach of breeding rapeseed meal glucosinolates degrading strain. |
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| Keywords: | rapeseed meal glucosinolates Aspergillus nigre N~ ion implantation mutation breeding |
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