| 平胸龟Sox基因的克隆和序列分析 |
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| 引用本文： | 聂刘旺,单祥年,郭超文.平胸龟Sox基因的克隆和序列分析[J].激光生物学报,2000,9(2):106-109. |
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| 作者姓名： | 聂刘旺 单祥年 郭超文 |
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| 作者单位： | 安徽师范大学生物系,中国安徽芜湖 |
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| 基金项目： | 安徽省和江苏省自然科学基金资助课题!( 974 1 2 0 0 2 and 9750 61 1 2) |
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| 摘 要： | 本文采用ＰＣＲ技术扩增和克隆了平胸龟的Ｓｏｘ基因，并通过银染测序进行了序列分析。结果显示，平胸龟雌雄个体均能扩增出相同长度的片段，其ＤＮＡ序列与人ＳＲＹ基因的ＨＭＧ－ｂｏｘ同源性达７２．７％，推测的氨基酸序列与ＳＲＹ基因的ＨＭＧ－ｂｏｘ序列同源性为４６．５％，充分显示出Ｓｏｘ基因在系统进化上的保守性。本文为Ｓｏｘ基因的起源和进化研究提供了资料。
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| 关 键 词： | Sox基因 序列分析 PCR 平胸龟 |
| 修稿时间： | 2000-02-02 |
The
Clone and Sequence Analysis of
Sox Gene in the P.megacephalum |
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| Abstract: | The Sox gene of P. megacephalum were amplified and clond using PCR. The amplification fragment was sequenced by PCR silversequencing. The results indicated that the Sox gene fragment can be amplified in P. megacephalum of male and female. The homologous of Sox gene sequences comparing with HMGbox of SRY is 72.7% , and the sequence homologous of diving amino acid is 46.5%. This may be concluded that Sox gene is conservative in phylogenetic. This paper provide the date for Sox gene study of origin and evolution. |
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| Keywords: | Sox gene Sequence analysis PCR |
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