| 细胞内微丝骨架的变化对人肝癌Bel-7402细胞形态的影响 |
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| 引用本文： | 林珏龙,陈耀文,李璟,方昆阳,许险峰,霍霞.细胞内微丝骨架的变化对人肝癌Bel-7402细胞形态的影响[J].激光生物学报,2004,13(3):224-227. |
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| 作者姓名： | 林珏龙 陈耀文 李璟 方昆阳 许险峰 霍霞 |
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| 作者单位： | 1. 汕头大学医学院中心实验室,中国广东,汕头,515031
2. 汕头大学医学院生物化学与分子生物学教研室,中国广东,汕头,515031 |
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| 摘 要： | 癌细胞的形态及癌细胞的生物学行为(如粘附、迁移和浸润)与细胞内骨架系统密切相关，本研究利用Phalloidin-FTTC标记人肝癌Bel-7402细胞内F-肌动蛋白(F-aetin)，使用激光扫描共聚焦显微镜观测细胞微丝骨架与癌细胞形态的关系，及Cyt-B处理Bel-7402细胞后，癌细胞内微丝骨架的变化。结果显示：癌细胞内F-aedn解聚，F-aetin小体的聚集重组是癌细胞的形态变化的主要因素之一。
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| 关 键 词： | 激光扫描共聚焦显微镜 人肝癌Bel-742细胞 F-肌动蛋白 微丝骨架 细胞松弛素-B |
| 文章编号： | 1007-7146(2004)03-0224-04 |
| 收稿时间： | 2003/11/20 |
| 修稿时间： | 2003年11月20 |
The Effect of Change in Cellular Microfilament Cytoskeleton on Cellular Morphology of Human Bel-7402 Hepatocarcinoma Cell |
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| LIN Jue-long,CHEN Yao-wen,LI Jing,FANG Kun-yang,XU Xian-feng,HUO Xia.The Effect of Change in Cellular Microfilament Cytoskeleton on Cellular Morphology of Human Bel-7402 Hepatocarcinoma Cell[J].ACTA Laser Biology Sinica,2004,13(3):224-227. |
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| Authors: | LIN Jue-long CHEN Yao-wen LI Jing FANG Kun-yang XU Xian-feng HUO Xia |
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| Institution: | LIN Jue-long~1,CHEN Yao-wen~1,LI Jing~2,FANG Kun-yang~1,XU Xian-feng~1,HUO Xia~1 |
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| Abstract: | Cellular morphology and cellular biological activities (i,e,. adhesion, mobility and invasion) are related to intracellular cytoskeleton. In the present study we used Phalloidin-FITC to label cellular F-actin of human Bel-7402 hepatocarcinoma cell. Intracellular microfilament of Bel-7402 cell and the change of cancer cellular microfilament cytoskeleton after Bel-7402 cell being treated with Cyt-B was observed by confocal scanning laser microscope (CSLM). The results showed that the morphological change of cancer cell was due to F-actin being depolymerized and actin protein being recombined to form the F-actin bodies. |
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| Keywords: | confocal scanning laser microscope (CSLM) human Bel-7402 hepatocarcinomacell F-actin microfilament cytoskeleton cytochalasin-B(Cyt-B) |
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