| CdSe量子点与蛋白质的作用研究 |
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| 引用本文： | 杨冬芝,孙世安,陈启凡,徐淑坤.CdSe量子点与蛋白质的作用研究[J].激光生物学报,2007,16(5):527-531. |
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| 作者姓名： | 杨冬芝 孙世安 陈启凡 徐淑坤 |
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| 作者单位： | 1. 东北大学化学系,辽宁,沈阳,110004
2. 徐州空军学院航空油料物资系,江苏,徐州,221000 |
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| 基金项目： | 国家自然科学基金
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高等学校博士学科点专项科研项目 |
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| 摘 要： | 以油酸为稳定剂,石蜡为还原剂,采用有机相法合成了尺寸均匀的CdSe量子点,并通过巯基乙酸将合成的量子点转移至水相。考查了CdSe量子点与几种结构不同的蛋白质(酶)之间的作用规律。研究发现,经巯基乙酸修饰后的量子点与牛血清白蛋白和胰凝乳蛋白酶作用后,荧光强度明显增大。而铜/锌-超氧化物歧化酶对量子点的荧光有明显的淬灭作用,牛血红蛋白对量子点的影响是随着时间的增加荧光强度先增大后减小,体现出一般蛋白质使荧光增强和部分金属离子使荧光淬灭两者的协同效应。
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| 关 键 词： | CdSe 量子点 蛋白质 酶 金属离子 |
| 文章编号： | 1007-7146（2007）05-0527-05 |
| 收稿时间： | 2006-11-13 |
| 修稿时间： | 2007-04-10 |
A Study on Interactions between Quantum Dots and Proteins |
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| YANG Dong-zhi,SUN Shi-an,CHEN Qi-fan,XU Shu-kun.A Study on Interactions between Quantum Dots and Proteins[J].ACTA Laser Biology Sinica,2007,16(5):527-531. |
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| Authors: | YANG Dong-zhi SUN Shi-an CHEN Qi-fan XU Shu-kun |
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| Institution: | 1. Department of Chemistry, Northeastern University, Shenyang 110004, Liaoning, China; 2. Department of Aviation Oil and Material, Xuzhou Air Force College, Xuzhou 221000, Jiangsu, China |
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| Abstract: | CdSe quantum dots(QDs) were synthesized in an organic system,in which paraffin and oleic acid acted as reducing and stabilizing reagent,respectively.Modified with 2-mercaptoacetic acid,the QDs were watersoluble which could be applied in bio-system.Their interactions between CdSe QDs and several proteins with different structures were studied.After conjugation with bovine serum albumin(BSA) and chymotrypsin,the fluorescence intensity of CdSe QDs was enhanced obviously and quenched after reacting with Cu/Zn-SOD.However,the fluorescence intensity of CdSe QDs was enhanced firstly,and then decreased with the increasing of reaction time with bovine hemoglobin.It embodied the coordinated effects of normal proteins and part of metal ions.The former could enhance its intensity,the latter could quench it. |
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| Keywords: | CdSe quantum dots protein enzyme metal ions |
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