氦氖激光对大鼠心肌超微结构、SOD和MDA影响的观察

为了解低强度氦氖激光对心肌细胞超微结构、超氧化物歧化酶（SOD）和丙二醛（MDA）的影响，采用2．87J／cm^2,7.17J/cm^2和28.66J/cm^2三种剂量氦氖激光照射大鼠心前区，电镜观察心肌超微结构，测定心肌组织SOD和MDA的含量。结果发现心肌线粒体有轻微损伤，心肌组织SOD含量在7．17J／cm^2剂量显著下降（P＜0．05），而在28．66J／cm^2剂量显著升高（P＜0．05），心肌组织MDA含量在7．17J／cm^2和28．66J／cm^2两种剂量下降有显著升高（P＜0．01），在2．87J／cm^2剂量，心肌组织SOD和MDA含量均无显著变化（P＜0．05）。结果提示心肌SOD含量和脂质过氧化随不同的激光剂量而变化。

Observation on Effect of He-Ne Laser Irradiation for Myocardiu mUltrastructure

In order to find out the effect of He-Ne laser irradiation on myocardium ultrastructure?superoxide dismutase(SOD) and malondialdehyde(MDA) in rats,the precordial regions of rats were separately irradiated by He-Ne laser with different doses(2.87?J/cm 2?7.17?J/cm 2?28.66?J/cm 2).Then,the ultrastructure of myocardium was studied,and the contents of SOD and MDA in myocardium were measured.The results showed that there was slight damage in mitochondria of myocardium,the content of MDA in myocardium tissue was significantly(P<0.01)increased with 7.17?J/cm 2 and 28.66?J/cm 2,the content of SOD in myocardium tissue was significantly(P<0.05)decreased with 7.17?J/cm 2 and increased with 28.66?J/cm 2(P<0.05).While no change of SOD and MDA content in myocardium tissue was observed with 2.87J/cm 2.The results suggest that the change of SOD content and lipid peroxidation in myocardium with the different laser doses.