| 牛Pbx-1基因的电子克隆及生物信息学分析 |
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| 引用本文： | 刘卉玲,崔群维,吴国良,范睿,蔡亚非,王根林.牛Pbx-1基因的电子克隆及生物信息学分析[J].激光生物学报,2009,18(1). |
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| 作者姓名： | 刘卉玲 崔群维 吴国良 范睿 蔡亚非 王根林 |
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| 作者单位： | 1. 安徽师范大学生物环境与生态安全安徽省高校重点实验室,安徽,芜湖,241000;安徽师范大学生物重要生物资源保护与利用研究重点实验室,安徽,芜湖,241000
2. 南京农业大学动物科技学院,江苏,南京,210095 |
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| 基金项目： | 国家科技支撑计划，芜湖市科技计划重点项目，安徽省自然科学基金 |
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| 摘 要： | 新基因全长cDNA序列很难获得,但电子克隆却提供了基因克隆的一种策略.利用小鼠Pbx-1基因编码序列(NM_183355)为种子序列进行电子克隆获得牛Pbx-1基因完整编码序列.然后,用生物信息学方法分析了牛的Pbx-1基因的结构,密码子偏性和氨基酸的同源性等.结果表明:该基因cDNA全长1 754 bp,无内含子,最大开放阅读框1 305 bp.编码434个氨基酸.预测其编码的蛋白分子量为47 189.5 Da,与小鼠的同源性为81%.
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| 关 键 词： | 牛Pbx-1基因 电子克隆 生物信息学 |
In Silico Cloning and Bioinformatics Analysis of the Cattle Pbx-1 Gene |
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| LIU Hui-ling,CUI Qun-wei,WU Guo-liang,FAN Rui,CAI Ya-fei,WANG Gen-lin.In Silico Cloning and Bioinformatics Analysis of the Cattle Pbx-1 Gene[J].ACTA Laser Biology Sinica,2009,18(1). |
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| Authors: | LIU Hui-ling CUI Qun-wei WU Guo-liang FAN Rui CAI Ya-fei WANG Gen-lin |
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| Institution: | 1.Anhui Normal University a.Key Laboratory of Biotic Environment and Ecological Safety in Anhui Province;b.Provincial Key Laboratory of Conservation and Exploitation of Biological Resources;Wuhu 241000;Anhui;China;2.Animal Science and Technology;College of Nanjing Agricultural University;Nanjing 210095;Jiangsu;China |
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| Abstract: | It is not easy to obtain the full-length cDNA sequence of a novel gene,however,in silico cloning provides a strategy for gene cloning.The complete coding sequence of cattle Pbx-1 gene was obtained by using mouse Pbx-1 gene coding sequence(NM_183355) as seeded sequence in silico cloning.Afterwards,the structure and codon adaptation of Pbx-1,and amino acid homology of Pbx-1 protein in cattle was analyzed by using bioinformatics methods.The results show that the cDNA of cattle Pbx-1 gene is 1 754 bp with no in... |
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| Keywords: | cattle Pbx-1 gene in silico cloning bioinformatics |
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