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TC-RT-PCR检测菜豆荚斑驳病毒的研究
引用本文:沈建国,高芳銮,廖富荣,王念武,郭琼霞,吴祖建.TC-RT-PCR检测菜豆荚斑驳病毒的研究[J].激光生物学报,2009,18(1).
作者姓名:沈建国  高芳銮  廖富荣  王念武  郭琼霞  吴祖建
作者单位:1. 福建出入境检验检疫局技术中心,福建省检验检疫技术研究重点实验室,福建,福州,350001;福建农林大学植物病毒研究所,福建,福州,350002
2. 福建农林大学植物病毒研究所,福建,福州,350002
3. 厦门出入境检验检疫局,福建,厦门,361012
4. 福建出入境检验检疫局技术中心,福建省检验检疫技术研究重点实验室,福建,福州,350001
基金项目:福建省自然科学基金,福建科技重大专项专题项目,福建出入境检验检疫局科技项目 
摘    要:根据已报道的菜豆荚斑驳病毒(Bean pod mottle virus,BPMV)外壳蛋白(Coat protein,CP)基因序列设计特异性引物,应用试管捕捉RT-PCR(Tube capture RT-PCR,TC-RT-PCR)技术对大豆种皮上的BPMV进行检测.结果表明,TC-RT-PCR方法能从携带BPMV的大豆种皮上扩增到预期大小的基因片段.将TC-RT-PGR扩增产物克隆测序后进行序列分析,结果显示扩增到的片段序列与BPMV的CP基因序列具有高度同源性,进一步证实了该方法的准确性.应用TC-RT-PCR方法,成功检测了一批进境大豆样品.本文建立的TC-RT-PCR方法,为大豆种子上BPMV的检测和诊断提供了一种新的参考方法.

关 键 词:菜豆荚斑驳病毒  检测

Detection of Bean pod mottle virus by TC-RT-PCR
SHEN Jian-guo,GAO Fang-luan,LIAO Fu-rong,WANG Nian-wu,GUO Qiong-xia,WU Zu-jian.Detection of Bean pod mottle virus by TC-RT-PCR[J].ACTA Laser Biology Sinica,2009,18(1).
Authors:SHEN Jian-guo  GAO Fang-luan  LIAO Fu-rong  WANG Nian-wu  GUO Qiong-xia  WU Zu-jian
Institution:1.Fujian Key Laboratory for Technology Research of Inspection and Quarantine;Technique Center of Fujian Exit-Entry Inspection and Quarantine Bureau;Fuzhou 350001;Fujian;China;2.Institute of Plant Virology;Fujian Agriculture and Forestry University;Fuzhou 350002;3.Xiamen Exit-Entry Inspection and Quarantine Bureau;Xiamen 361012;China
Abstract:A pair of specific primers were designed and synthesized based on the conserved nucleotide sequence of coat protein(CP) gene of Bean pod mottle virus(BPMV).Tube capture RT-PCR(TC-RT-PCR) was established to detect BPMV in soybean seed coat.The results showed that the target fragment could be amplified from the BPMV-infected soybean seed coats.Cloning and sequencing analysis indicated that nucleotide sequence of the target fragment was highly identical to the reported BPMV genomic sequence,which verified the ...
Keywords:TC-RT-PCR
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