| MTT法和CCK-8法检测细胞活性之测试条件比较 |
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| 引用本文： | 熊建文,肖化,张镇西.MTT法和CCK-8法检测细胞活性之测试条件比较[J].激光生物学报,2007,16(5):559-562. |
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| 作者姓名： | 熊建文 肖化 张镇西 |
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| 作者单位： | 1. 华南师范大学物理与电信工程学院,广东,广州,510006
2. 西安交通大学生物医学信息工程教育部重点实验室,生命科学与技术学院,陕西,西安,710049 |
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| 基金项目： | 国家自然科学基金
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广东省教育厅自然科学基金 |
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| 摘 要： | 利用HL60作为研究对象,对比了MTT法和CCK-8法在最佳检测时刻和检测波长等参数的差异。对于CCK-8法,最佳测试时刻在细胞液培养4 h后,最佳测试波长为490 nm。实验结果表明用CCK-8法检测的OD值与活细胞数的线性相关度要优于MTT法,是一种灵敏度较高、重复性好的的细胞活性检测方法。
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| 关 键 词： | MTT CCK-8 标定曲线 细胞活性 |
| 文章编号： | 1007-7146（2007）05-0559-04 |
| 收稿时间： | 2006-12-04 |
| 修稿时间： | 2007-05-08 |
An Experimental Research on Different Detection Conditions between MTT and CCK-8 |
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| XIONG Jian-wen,XIAO Hua,ZHANG Zhen-xi.An Experimental Research on Different Detection Conditions between MTT and CCK-8[J].ACTA Laser Biology Sinica,2007,16(5):559-562. |
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| Authors: | XIONG Jian-wen XIAO Hua ZHANG Zhen-xi |
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| Abstract: | The different detection conditions based on HL60 between MTT and CCK-8 were studied.The best test time is 4 h,the best test wavelength is 490 nm for CCK-8.The linearity of CCK-8 between OD and cell viability was better than those of MTT.CCK-8 was a kind of cell viability assay reagent with a higher sensitivity and a better reproducibility. |
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| Keywords: | MTT CCK-8 calibration curves cell viability |
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