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聚乙二醇对栝楼种子萌发的影响
引用本文:何家庆,黄训端,黎承姬,施忠辉.聚乙二醇对栝楼种子萌发的影响[J].激光生物学报,2005,14(6):416-419.
作者姓名:何家庆  黄训端  黎承姬  施忠辉
作者单位:安徽大学生命科学学院,中国安徽,合肥,230039;安徽省生态工程与生物技术重点实验室,中国安徽,合肥,230039;安徽大学生命科学学院,中国安徽,合肥,230039;安徽省绩溪县政府,中国安徽,绩溪,245300
基金项目:安徽省星火计划(04053051)
摘    要:针对栝楼籽萌发困难的问题,本试验采用聚乙二醇(PEG)对栝楼种子进行处理,结果表明:15%PEG浸种24h,可有效调节渗透水势,改变种子生理生化反应特性,并保持淀粉酶活性连续上升,使可溶性蛋白质含量明显提高,可见PEG在加速大分子动员和提高代谢能力方面发挥了作用,从而促进植物体的组织器官形态建成和生长发育;同时,PEG处理提高了过氧化物酶(POD)活性,降低了丙二醛(MDA)含量,减少了自由基积累,减轻了浸种吸涨对膜系统的损伤,降低了不利因素的影响,提高了种子活力。

关 键 词:聚乙二醇  栝楼  种子  萌发
文章编号:1007-7146(2005)06-0416-04
收稿时间:2005/3/1
修稿时间:2005年3月1日

Effects of PEG ( Polyethylene Giyco) on Seeds Germination of richosanthes kirilowii
HE Jia-qing,HUANG Xun-duan,LI Cheng-ji,SHI Zhong-hui.Effects of PEG ( Polyethylene Giyco) on Seeds Germination of richosanthes kirilowii[J].ACTA Laser Biology Sinica,2005,14(6):416-419.
Authors:HE Jia-qing  HUANG Xun-duan  LI Cheng-ji  SHI Zhong-hui
Institution:HE Jia-qing 1,2,HUANG Xun-duan 1,2,LI Cheng-ji1,SHI Zhong-hui3
Abstract:In this paper,15% PEG was applied to soak the seeds of Trichosanthes kirilowii for 24 h in order to promote its germination.The results show that PEG could decrease osmotic pressure of water and change reactions of physiology and biochemistry in seeds effectively.For example,PEG could bring the high activity of amylase consecutively,and increase the content of dissoluble protein and the activity of POD (Peroxidase), reduce accumulation of free radical, but decrease the content of MDA (Malondialdehyde) in seeds. Based on the experiment it might be said that PEG pretreatment could improve metabolic activity and make cell membrane system less damage in the course of absorbing water in seed,too.All these indicate that PEG can increase seed vitality and germination capacity.
Keywords:PEG  Trichosanthes kirilowii  seeds  germination
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