运用代谢组学研究RSV与水稻的互作初探

水稻条纹叶枯病严重威胁着我国的水稻生产，该病由水稻条纹病毒（Rice stripe virus，RSV）引起。目前，对水稻与RSV互作机制的认识还较少，这是制定有效措施来对RSV进行防控的一大障碍。代谢组学是新近发展起来的一种研究手段，它通过考察生物体系包括细胞、组织或整个生物体在受到刺激或扰动后，在代谢水平上的应答，来研究一系列的生物现象。运用代谢组学对水稻与RSV的互作进行了初步探索。GC-MS分析表明，RSV的侵染能显著影响水稻的代谢谱，且在代谢组水平上，不同抗感性的水稻对RSV的响应存在着差异。与NIST质谱数据库中的信息比较，从武育粳3号健康植株中鉴定到内源性代谢物12种、武育粳3号发病植株中11种、KT95-418健康植株中9种、KT95-418发病植株中14种。

A Pilot Study of Metabolites in Rice Stripe Virus Infected Rice(Oryza Sativa L.)

Rice stripe disease caused by rice stripe virus(RSV) is a major threat to the rice production of China.One of the most important hurdles preventing the development of effective controlling measures of RSV is the lack of knowledge on RSV-rice interaction.Metabolomics is a newly developed research area which studies the responses of a cell,a tissue or the whole body to environmental cues at the level of metabolites.RSV infection could result in significant changes in the metabolites of rice by GC-MS.At the level of metabolites,the rice cultivars with distinct susceptibility might respond differentially to RSV.By database retrieving,12,11,9 and 14 kinds of metabolites were identified from Wuyujing 3 healthy,Wuyujing 3 diseased,KT95-418 healthy and KT95-418 diseased plants,respectively.