低功率He-Ne激光照射下细胞内活性氧自由基的产生和游离Ca^2＋浓度的研究

在临床应用中,低功率He-Ne激光(632.8 nm)能促进骨骼肌修复,加速创伤愈合,降低牙齿的超敏感性,减缓疼痛等.大量研究表明:低功率He-Ne激光能调节细胞的众多行为,如细胞增殖、分泌、迁移、粘附、蛋白质合成和基因表达等.但低功率He-Ne激光调节细胞行为的分子机制并未阐明,考察低功率He-Ne激光照射后细胞内活性氧自由基的产生水平和游离ca2 浓度是否会发生变化,通过激光扫描共聚焦显微镜,分别利用H:DCFDA和Fluo-3/AM这两种荧光探针,检测到经He-Ne激光照射后,肺腺癌细胞内活性氧自由基的水平上调以及游离Ca2 浓度增加.该研究为低功率He-Ne激光的生物光刺激效应提供了可能的分子机理.

Research on the Generation of ROS and Intracellular Calcium Concentration Induced by Low Power He-Ne Laser Irradiation

Low power He-Ne laser(632.8 nm) irradiation have been applied clinically to promote skeletal muscle regeneration,accelerate wound healing,decrease dental hypersensitivity,relieve pain,and so on.Many researches indicate that low power He-Ne laser irradiation could modulate various biological processes such as promoting cell proliferation,secretion,migration,adhesion,protein synthesis,gene expression.However,the mechanism of photo-stimulative effect has not been fully clarified.In this study,we investigated whether low power He-Ne laser irradiation induced the generation of ROS and the increase of Ca2 .Using two fluorescence probes H2DCFDA and Fluo-3/AM,we found that the generation of ROS and the release of Ca2 were induced in the human lung adenocarcinoma cells(ASTC-a-1) by 0.8 J/cm2 He-Ne laser irradiation observed with the laser scanning confocal microscope(LSM).Our study provided a possible molecular mechanism for the photo-biostimulative effect of low power He-Ne laser irradiation.