He-Ne激光对啤酒大麦麦芽同工酶基因表达的影响

采用不同时间He-Ne激光（5 m W/mm2）对＂临啤二号＂大麦种子进行辐照,利用聚丙烯酰胺凝胶电泳（PAGE）技术分析激光辐照对大麦麦芽中超氧化物歧化酶（SOD）同工酶、过氧化物（POD）同工酶、过氧化氢（CAT）同工酶、β-淀粉酶同工酶、三磷酸腺苷（ATPase）同工酶酶谱的影响。结果表明：种子经He-Ne激光辐照后诱导出1条新POD同工酶谱带,且总POD活性在60 s和90 s处有所增加;30 s激光辐照诱导出1条新的CAT同工酶谱带,其它时间处理酶的组分没有变化,但酶活性增加;He-Ne激光辐照使SOD、β-淀粉酶及ATPase同工酶的组分在不同时间下有所减少,但一定时间的激光辐照提高了其同工酶的活性。综合实验结果可知,60 s～90 s He-Ne激光可以促进大麦种子萌发过程中以上同工酶基因的表达,有利于缩短制麦周期,提高麦芽糖化力及抗氧化力。

Effects of He-Ne Laser on the Isozyme Expression of Beer Barley

The seeds of a beer barley cultivar（Linpi No. 2） were exposed to He-Ne laser irradiation（5 m W / mm2）with varied duration,the polyacrylamide gel electrophoresis（PAGE） was used to analyze the patters of superoxide dismutase（SOD）,peroxidase（POD）,catalase（CAT）,β-amylase and adenosine triphosphatase（ATPase） in malt. The results showed that one new band of POD zymogram appeared and the activities of total POD enhanced in 60 s and 90 s radiation groups after He-Ne laser irradiation compared with the control; as for CAT,it showed that one new band of CAT zymogram appeared in 30 s radiation group and the activities of total CAT enhanced though there are no isozyme bands changing in other groups; there were isozyme bands disappearing in SOD,β-amylase and ATPase in different radiation groups compared to that of control,but the activities of the isozymes increased after certain duration radiation. To sum up,60 s ~ 90 s irradiation promoted the expression of above isozyme genes which might shorten the malting process and improve the malt diastatic power and antioxidant capacity.