| 人膀胱癌细胞纤维肌动蛋白共聚焦激光扫描显微镜观察 |
| |
| 引用本文： | 许险峰,霍霞,杨海伟,陈耀文,林珏龙.人膀胱癌细胞纤维肌动蛋白共聚焦激光扫描显微镜观察[J].激光生物学报,2002,11(3):202-206. |
| |
| 作者姓名： | 许险峰 霍霞 杨海伟 陈耀文 林珏龙 |
| |
| 作者单位： | 汕头大学医学院中心实验室,中国广东汕头,515031 |
| |
| 摘 要： | 探讨膀胱癌细胞纤维肌动蛋白（F－actin)的空间结构。采用共聚焦激光扫描显微镜光学切片技术结合异硫酸氢荧光素－鬼笔环肽（FITC－phalloidin)标记纤维肌动蛋白和碘化丙啶（PI）和标记核酸的荧光探针双重标记技术对膀胱癌细胞纤维肌动蛋白进行形态学观察，结果可见膀胱癌细胞内纤维肌动蛋白微丝形态完整，成细束或细丝状，平行排列地整个细胞或细胞突起，在胞质边缘处较密集。
|
| 关 键 词： | 人膀胱癌细胞 纤维肌动蛋白 共聚焦激光扫描 F－actin 光学切片 |
| 文章编号： | 1007-7146(2002)03-0202-05 |
| 收稿时间： | 2002/3/11 |
| 修稿时间： | 2002年3月11日 |
Imaging of F-actin in Bladder Cancer Cells with Confocal Laser Scanning Microscope |
| |
| XU Xian-feng,HUO Xia,YANG Hai-wei,CHEN Yao-wen,LIN Jue-long.Imaging of F-actin in Bladder Cancer Cells with Confocal Laser Scanning Microscope[J].ACTA Laser Biology Sinica,2002,11(3):202-206. |
| |
| Authors: | XU Xian-feng HUO Xia YANG Hai-wei CHEN Yao-wen LIN Jue-long |
| |
| Abstract: | To study the spatial structure of F-actin in bladder cancer cells.F-actin marked by FITC-phalloidin and nucleic acid marked by PI in bladder cancer cells were observed by confocal laser scanning microscope to generate the optical sections,then analyzed by image-analysis procedure of confocal laser scanning microscope.The results show that in bladder cancer cells F-actin was filaceous or bunchy,ranging parallelly through the whole cell or cellular prominences and dense on the edge of cytoplasm. |
| |
| Keywords: | bladder cancer cells confocal laser scanning microscope F-actin optical sections |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
