| pLXSN-MICA~* 008和pLXSN-MICA~* 001在人成纤维细胞表达 |
| |
| 引用本文： | 刘立民,张琛涛,罗奇志,霍治,林琳,杜昆,王芙艳,余平.pLXSN-MICA~* 008和pLXSN-MICA~* 001在人成纤维细胞表达[J].激光生物学报,2012,21(2):150-155. |
| |
| 作者姓名： | 刘立民 张琛涛 罗奇志 霍治 林琳 杜昆 王芙艳 余平 |
| |
| 作者单位： | 1. 中南大学基础医学院免疫学系,湖南长沙,410078
2. 广西省桂林医学院附属医院心血管内科,广西桂林,541000 |
| |
| 基金项目： | 国家自然科学基金项目，湖南省自然科学基金，中南大学研究生教育创新课题项目 |
| |
| 摘 要： | 目的:建立表达MICA* 008和MICA* 001蛋白的人包皮成纤维(human foreskin fibroblast,HFF)细胞,为研究MICA基因的免疫功能提供生物学材料。方法:组织块法原代培养HFF细胞;用脂质体将质粒pLXSN,pLXSN-MICA* 008和pLXSN-MICA* 001转染PA317包装细胞后收集病毒颗粒,再将病毒颗粒感染HFF细胞,G418筛选14天,扩增耐药细胞,westernblot检测MICA* 008和MICA* 001蛋白表达情况。结果:逆转录病毒载体pLXSN,pLXSN-MICA* 008和pLXSN-MICA* 001经包装细胞PA317包装可产生有感染能力的病毒,该病毒感染HFF细胞后,westernblot检测出外源蛋白MICA* 008和MICA* 001。结论:建立了表达MICA* 008和MICA* 001蛋白的人成纤维细胞。
|
| 关 键 词： | pLXSN-MICA* 008 pLXSN-MICA* 001 成纤维细胞 |
Expression of Gene pLXSN-MICA*008 and pLXSN-MICA*001 in Human Foreskin Fibroblast Cell |
| |
| LIU Limin
,
ZHANG Chentao
,
LUO Qizhi
,
Huo zhi
,
LIN Lin
,
DU Kun
,
WANG Fuyan
,
YU Ping.Expression of Gene pLXSN-MICA*008 and pLXSN-MICA*001 in Human Foreskin Fibroblast Cell[J].ACTA Laser Biology Sinica,2012,21(2):150-155. |
| |
| Authors: | LIU Limin ZHANG Chentao LUO Qizhi Huo zhi LIN Lin DU Kun WANG Fuyan YU Ping |
| |
| Institution: | 1. ( 1. Department of Immunology, Basic Medical College, Central South University, Changsha 410078, Hunan, China; 2. Department of Cardiovascular Internal Medicine of Affiliated Hospital, Guilin Medical College, Guilin 541000, Guangxi, China) |
| |
| Abstract: | Objective ： Construct human foreskin fibroblast （HFF） cell expression protein MICA ＊ 008, MICA ＊ 001 to provide biotic material to research immune function of gene MICA. Methods ： Cell culture in vitro technique was used ; The retroviral vector pLXSN, pLXSN-MICA ＊ 008 and pLXSN-MICA ＊ 001 transfect packaging cell PA317 with lipo- somes to gain virus particles. HFF cells are infected by virus particles and screened with G418 resistance for 14 days. The expression protein MICA ＊ 008, MICA ＊ 001 were dentified by westerublot. Results：Packaged by packaging cell, the ret- roviral vector pLXSN, pLXSN-MICA ＊ 008 and pLXSN-MICA ＊ 001 form virus particles with infection ability which are able to infect HFF cell and protein MICA ＊ 008, MICA ＊ 001 was expressed in HFF cell indicated by westernblot. Conclusion： HFF cell expression protein MICA ＊ 008, MICA ＊ 001 was constructed successfully. |
| |
| Keywords: | pLXSN-MICA ＊ 008 pLXSN-MICA ~ 001 foreskin fibroblast |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
