六种灵猫科动物的分子系统关系研究

对六种灵猫科物种线粒体12 S rRNA基因及其中四种的Cytb基因部分序列进行了测定,并从Gen-Bank获得斑林狸(Prionodon pardicolor)、熊狸(Arctictis binturong)的Cytb基因同源序列。两基因整合序列比对后长755 bp,12 S rRNA基因序列中有70个变异位点,31个简约信息位点,在Cytb基因序列中,共有120个位点呈现变异,60个简约信息位点,Cytb基因的碱基变异百分比高于12 S rRNA基因的碱基变异百分比。使用邻接法(NJ)、最大似然法(ML)重建的分子系统树显示:斑林狸从灵猫亚科中分离出来,支持灵猫亚科的多系起源,而且斑林狸可能是中国起源最早且最特化的灵猫科动物。另外,同属于灵猫亚科的大灵猫(Viverra zibe-tha)、小灵猫(Viverricula indica)聚为一支,同属于棕榈狸亚科的果子狸(Viverricula indica)、熊狸聚为姐妹群,这些与传统形态学分类观点一致。

Phylogenetic Relationship Analysis of 6 Viverridae Species Using Mitochondrial Sequences

Partial sequences of mitochondrial 12 S rRNA gene from 6 civets and Cyt b gene sequences from 4 species were sequenced.The combined data was 755 bp long,containing 191 variable sites,90 parsimony informative sites.There were 120 variable sites in Cyt b gene sequence(375 bp),and it showed higher nucleotide variation(32 %) than 12 S rRNA gene(370 bp),which only had 70 variable sites(18.4 %).Molecular phylogenetic tree(NJ and ML) supported that Viverrine was polyphyletic,and implied spotted linsang(Prionodon pardicolor) perhaps was basal and mostly specified to other Viverridae species in China.Meanwhile,large Indian civet(Viverra zibetha),small Indian civet(Viverricula indica) clustered together,masked palm civet(Viverricula indica),binturong(Arctictis binturong) clustered together and they constituted monophyletic group respectively.These were consistent with traditional taxonomy arrangement.