| 激素水平对84 K杨组培快繁的影响 |
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| 引用本文： | 张静,林毅,蔡永萍,厉荣玉,魏凤娟.激素水平对84 K杨组培快繁的影响[J].激光生物学报,2005,14(3):233-237. |
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| 作者姓名： | 张静 林毅 蔡永萍 厉荣玉 魏凤娟 |
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| 作者单位： | 安徽农业大学生命科学学院,中国,安徽,合肥,230036 |
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| 基金项目： | 安徽省人才开发资金资助项目(2003526) |
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| 摘 要： | 本实验以84K杨腋芽和叶片为外植体，研究不同激素水平对84K杨组织培养的影响，探讨84K杨快速繁殖试管苗的有效途径。研究结果表明：84K杨腋芽增殖的最佳培养基为MS 6-BA2．0mg／L NAA0．5mg/L；叶片不定芽诱导初期的最佳培养基为MS 6-B1．0mg／L NAA0．5mg/L，MS KT1．0mg／L NAA0．1mg，L为不定芽壮苗的最佳培养基；从生根和移栽成活率上看不添加任何激素的1／2MS基本培养基和1/2MS NAA0．2mg/L IBA0．5mg/L为84K杨最佳生根培养基。
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| 关 键 词： | 84K杨 激素水平 组织培养 |
| 文章编号： | 1007-7146(2005)03-233-05 |
| 收稿时间： | 2005/3/10 |
| 修稿时间： | 2005年3月10日 |
Effect of Hormone on Tissue Culture and Fast Propagation of Poplar 84 K |
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| ZHANG Jing,LIN Yi,CAI Yong-ping,LI Rong-yu,WEI Feng-juan.Effect of Hormone on Tissue Culture and Fast Propagation of Poplar 84 K[J].ACTA Laser Biology Sinica,2005,14(3):233-237. |
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| Authors: | ZHANG Jing LIN Yi CAI Yong-ping LI Rong-yu WEI Feng-juan |
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| Institution: | ZHANG Jing,LIN Yi~*,CAI Yong-ping,LI Rong-yu,WEI Feng-juan |
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| Abstract: | The paper reported the effect of different hormone content on tissue culture of poplar 84 K with the axillary shoots and leaves as explantlets, discussed the available methodology on rapid propagation clone plplar 84 K. The results indicated as follow: The best medium of axillary shoots induction is MS+6-BA 2.0 mg/L+ NAA 0.5 mg/L; the best medium of bud differentiation from lamina on first time is MS+6-B 1.0 mg/L+NAA 0.5 mg/L,and MS+KT 1.0 mg/L+NAA 0.1 mg/L is the best medium of bud; according to rooting and survival rate, 1/2 MS without any hormones and 1/2 MS+NAA 0.2 mg/L+ IBA 0.5 mg/L is the best medium of rooting. |
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| Keywords: | poplar 84 K hormone content tissue culture |
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