| 低能离子注入L-乳酸生产菌种选育与发酵条件初步优化 |
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| 引用本文： | 虞龙,周剑,余增亮.低能离子注入L-乳酸生产菌种选育与发酵条件初步优化[J].激光生物学报,2005,14(3):184-188. |
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| 作者姓名： | 虞龙 周剑 余增亮 |
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| 作者单位： | 1. 南京工业大学制药与生命科学学院,中国,江苏,南京,210009
2. 中国科学院离子束生物工程学重点实验室,中国,安徽,合肥,230031 |
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| 基金项目： | 江苏省高校自然科学研究计划(03KJB180038) |
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| 摘 要： | 通过20keV氮离子注入L-乳酸生产菌(Bacillus coagulans)筛选到一株产量比出发菌株提高10％的高产菌株RS12-6C，经多次传代实验表明该菌遗传稳定性较好。并对其发酵条件如接种量、装液量、摇床转速、温度等进行初步优化，在含糖150g/L的摇瓶中发酵，其L-乳酸产量达到117g/L。
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| 关 键 词： | 离子注入 诱变 选育 L-乳酸 |
| 文章编号： | 1007-7146(2005)03-184-05 |
| 收稿时间： | 2004/11/30 |
| 修稿时间： | 2004年11月30 |
Screening of L-lactic Acid Producing Bacteria by Low Energy Ions Implantation and Preliminary Research of Fermentation Conditions |
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| YU Long,ZHOU Jian,YU Zeng-liang.Screening of L-lactic Acid Producing Bacteria by Low Energy Ions Implantation and Preliminary Research of Fermentation Conditions[J].ACTA Laser Biology Sinica,2005,14(3):184-188. |
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| Authors: | YU Long ZHOU Jian YU Zeng-liang |
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| Institution: | YU Long~1,ZHOU Jian~1,YU Zeng-liang~2 |
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| Abstract: | Through 20 keV N~+ implanting into L-lactic acid producing bacteria Bacillus coagulans, a high-yield mutant strain RS12-6C was obtained. Its lactic acid yield was increased by 10% than that of original. The culturing conditions such as pH control, oxygen supply and temperature was also studied deliberately. And the optimal carbon and nitrogen source and their optimal sugar concentration etc,were determined. The concentration of L-lactic acid reached to 117 g/L under the condition of 150 g/L glucose in fermentation medium. |
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| Keywords: | ion implantation mutation breeding L-lactic acid |
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