| 蜜环菌多糖对低能离子束诱变作用的恢复效应 |
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| 引用本文： | 张颖,沈业寿,葛继志,尹若春,孔小卫.蜜环菌多糖对低能离子束诱变作用的恢复效应[J].激光生物学报,2002,11(4):272-275. |
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| 作者姓名： | 张颖 沈业寿 葛继志 尹若春 孔小卫 |
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| 作者单位： | 安徽大学生命科学学院,中国安徽合肥,230039 |
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| 基金项目： | 安徽省教委重点资助项目 (98JL 0 2 9Z)，安徽省教委资助项目 (2 0 0 1KJ0 11) |
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| 摘 要： | 水提法从密环菌Am99-1菌株人工发酵体分离得到密环菌多糖Aml，以低能N^ 离子束为诱变源，黑腹果蝇为受试动物，检测Aml对离子束诱变果蝇的恢复功能。实验结果经统计学分析表明，1.0%和1.5%浓度的密环菌多糖Aml可以提高受试果蝇蛹的孵出率和降低当代及F2代果蝇突变率。这说明密环菌多糖对于低能离子束诱变有一定程度的防护作用。染色体水平上的检测也证实了此结论。
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| 关 键 词： | 蜜环菌 多糖 抗突变 黑腹果蝇 |
| 文章编号： | 1007-7146(2002)04-0272-04 |
| 收稿时间： | 2002/3/27 |
| 修稿时间： | 2002年3月27日 |
Reverse Effects of Polysaccharide from Armillaria mellea Mutated by N+ Ion Beam Implantation |
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| ZHANG Ying,SHEN Ye shou,GE Ji zhi,YIN Ruo chun,KONG Xiao wei.Reverse Effects of Polysaccharide from Armillaria mellea Mutated by N+ Ion Beam Implantation[J].ACTA Laser Biology Sinica,2002,11(4):272-275. |
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| Authors: | ZHANG Ying SHEN Ye shou GE Ji zhi YIN Ruo chun KONG Xiao wei |
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| Abstract: | A polysaccharid named Am1 was isolated by water extraction from mycelium of Armillaria mellea . Two dosage of N + ion beam radiated Dnosophila melanogaster feeded with three concentration Am1 and the resistance to the resulting mutation was tested. According to the statistical analysis, the experimental result showed Am1 at the concentration of 1.0% and 1.5% had improved the incubated pupae percentage and decreased the rate of mutation in parent and filial generation. Therefore, the conclusion that Am1 has the function of antimutagenicity was obtained. Furthermore, the detection on the level of chromosome has also proved it. |
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| Keywords: | Armillaria mellea polysaccharid antimutagenicity D melanogaster |
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