| 酸性品红与牛血清白蛋白变色反应机理的研究 |
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| 引用本文： | 曹稳根,赵海泉.酸性品红与牛血清白蛋白变色反应机理的研究[J].激光生物学报,2006,15(5):501-505. |
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| 作者姓名： | 曹稳根 赵海泉 |
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| 作者单位： | 1. 安徽农业大学生命科学学院,安徽,合肥,230036;宿州学院化生系,安徽,宿州,234000
2. 安徽农业大学生命科学学院,安徽,合肥,230036 |
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| 摘 要： | 利用光谱探针技术研究酸性品红(fuchsine ac id,FA)与牛血清白蛋白(bovine serum album in,BSA)的变色反应机理,考察了不同实验条件对FA-BSA复合物吸收光谱的影响。实验结果表明,FA与BSA分子相互作用产生变色反应的机理主要是由FA与BSA间的疏水相互作用引起,而静电作用则是形成FA-BSA桃红色复合物的必要条件。
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| 关 键 词： | 酸性品红 牛血清白蛋白 光谱探针 变色反应机理 |
| 文章编号： | 1007-7146(2006)05-0501-05 |
| 收稿时间： | 2005/11/17 |
| 修稿时间： | 2005年11月17 |
Study on the Mechanism of Color Changes between Fuchsine Acid and Bovine Serum Albumin |
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| CAO Wen-gen,ZHAO Hai-quan.Study on the Mechanism of Color Changes between Fuchsine Acid and Bovine Serum Albumin[J].ACTA Laser Biology Sinica,2006,15(5):501-505. |
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| Authors: | CAO Wen-gen ZHAO Hai-quan |
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| Abstract: | The mechanism of color changes between fuchsine acid(FA) and bovine serum albumin(BSA) was studied by spectra probe.And the influence of different experimental conditions on the absorption spectra of FA-BSA mixtures was investigated.Through these experiments,one would draw the conclusion that the color changes of FA-BSA complex results mainly from the hydrophobic interaction between FA and BSA while the electrostatic interaction is still necessary for the formation of pink FA-BSA complex. |
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| Keywords: | fuchsine acid bovine serum albumin spectra probe mechanism of color changes |
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