鲤的微卫星引物对草鱼基因组分析适用性的初步研究

运用微卫星DNA-聚合酶链反应(STR—PCR)基因分型技术，选取已发表的28对鲤的微卫星引物，探讨鲤的引物用于草鱼基因组微卫星分析的可能性。通过优化PCR反应条件，消除了影子带和异源核酸双链分子两类微卫星相关假阳性带对STR—PCR分析的干扰。在此基础上，筛选出7对引物可在湘江野生草鱼基因组中扩增出特异性条带，占总数的25％；其中的4对引物(约占总数的14．3％)在8尾湘江野生草鱼小群体中即检测到了个体间等位基因的多态性。这些初步的结果表明鲤的微卫星引物可以用于草鱼基因组的分析。

Preliminary Study on Applicability of Microsatellite Primers Developed from Common Carp for Genomic Analysis of Grass Carp

In order to determine the applicability of microsatellite primers developed from common carp (Cyprinus carpio) for genomic analysis in grass carp (Ctenopharyngodon idellus), twentyeight pairs of common carp primers designed for microsatellites containing CA motifs were employed to amplify the microsatellite loci in the genome of grass carp. The conditions of polymerase chain reaction (PCR) were optimized for the fidelity of DNA synthesis during PCR amplification. Two kinds of nonspecific PCR products, heteroduplex bands and shadow bands, were eliminated successfully by decreasing the extension temperature and Mg2+ concentration. 7 primers (about 25%) amplified specific products successfully and 4 primers (about 14.3%) have shown the polymorphism in a small population of the wild grass carp of xiangiang river (only 8 samples of fish). This result indicates that there are about 50% homology of sequences flanking the microsatellite loci between the common carp and grass carp, and some of the commonon carp and grass carp,and some of the common carp microsatellite primers can be used for grass carp genetic analysis without much costing and time consuming.