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| 60Coγ射线诱变褐色高温单孢菌及其在粪便发酵中的应用研究 | |
| 引用本文： | 毛湘冰,肖兵南,刘毅,杨爱芝,周望平,邱美珍.60Coγ射线诱变褐色高温单孢菌及其在粪便发酵中的应用研究[J].激光生物学报,2006,15(3):316-319. |
| 作者姓名： | 毛湘冰 肖兵南 刘毅 杨爱芝 周望平 邱美珍 |
| 作者单位： | 1. 湖南省畜牧兽医研究所,湖南,长沙,410131;湖南农业大学动物科技学院,湖南,长沙,410128 2. 湖南省畜牧兽医研究所,湖南,长沙,410131 3. 湖南农业大学动物科技学院,湖南,长沙,410128 4. 湖南省宁乡县白马桥乡农技站,湖南,宁乡,410600 |
| 基金项目： | 2004年湖南省科技重大专项(04NK1006) |
| 摘 要： | 本试验以褐色高温单孢菌(Therm om onospora fusca)为出发菌株,通过60Coγ射线诱变孢子悬液,采用透明圈法初筛和摇瓶培养复筛的方法,获得了一株纤维素酶高产菌株AV5,与出发菌株相比,其产酶能力提高1.8倍。接种牛粪发酵后,牛粪中粗纤维含量降低率为32.95%,是接种出发菌株相对降解率的1.7倍。 |
| 关 键 词： | 褐色高温单孢菌 纤维素酶 60Coγ射线诱变 粪便发酵 |
| 文章编号： | 1007-7146（2006）03-0316-04 |
| 收稿时间： | 2005-07-20 |
| 修稿时间： | 2005-09-19 |
The Studies on Mutagenesis of Thermomonospora fusca with 60 Co Gamma Rays and its Application in the Fermentation of Excrement |
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| MAO Xiang-bing,XIAO Bing-nan,LIU Yi,YANG Ai-zhi,ZHOU Wang-ping,QIU Mei-zhen.The Studies on Mutagenesis of Thermomonospora fusca with 60 Co Gamma Rays and its Application in the Fermentation of Excrement[J].ACTA Laser Biology Sinica,2006,15(3):316-319. | |
| Authors: | MAO Xiang-bing XIAO Bing-nan LIU Yi YANG Ai-zhi ZHOU Wang-ping QIU Mei-zhen |
| Institution: | 1. Hunan Institute of Animal and Veterinary Science, Changsha 410131, Hunan, China; 2. College of Animal and Sciences-Technology , Hunan Agricultural University, Changsha 410128, Hunan, China; 3. The Agricultural Technology Stage in Baima Bridge Village, Ningxiang County, Hunan Province, Ningxiang 410600, Hunan, China |
| Abstract: | In this experiment,Thermomonospora fusca was chosen as an original strain for screening high yield cellulase mutants.The spore suspension of organism was treated with ~(60)Co gamma rays.A high cellulase-producing mutant strain AV5 was obtained by screening using the methods of transparent zone and shaking culture.The activity of mutant strain AV5 was enhanced for 1.8 times comparing with that of the original strain.After fermentation the excrement of cattle,the reducing rate of the crude cellulose of excrement is 32.95 %,which is 1.7 times higher than the reducing rate of the original strain. |
| Keywords: | Thermomonospora fusca cellulase mutantion of ~(60)Co gamma rays the fermentation of excrement |
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