哈茨木霉发酵液中肽类物质对豇豆根瘤结构和功能的影响

通过树脂吸附、离子交换、薄层层析、高效液相色谱系统等分离、纯化方法,从哈茨木霉(Trichoderm aharzianum)T2-16菌株发酵液中分离得到一种对豆科作物生长具促进作用的物质,通过质谱等方法鉴定为肽类物质。为探明该活性物质对豆科作物的促生机制,用该活性物质对豇豆种子浸种处理后,进行盆栽实验,通过对盆栽实验中根瘤结构与固氮活性变化的研究,结果显示,该活性物质可增加根瘤侵染组织的面积和根瘤细胞中类菌体的数量,降低根瘤细胞液泡化程度,促进根瘤中公共细胞周膜较多形成,加快类菌体的发育成熟,提高根瘤豆血红蛋白的含量,从而提高根瘤的固氮活性。

Effects of Peptide in the Fermentation Liquid of Trichoderma harzianum on Nodule Microstructure and Function of Cowpea

Various methods including multiple column layers,TLC,HLPC,UV and MS were adopted in the isolation,purification and identification of the growth promoting substance in the fermented liquid of Trichoderma harzianum T_(2-16).The test result suggested the growth promoting substance was a kind of peptide.The mechanism of growth promoting substance was studied.The effects on nodule microstructure and nitrogen-fixing of cowpea were studied through it was applied by seed soaking in greenhouse pot tests.The results is as follows: The ratio of center tissue(tissue infected) and the cells in the nodules of cowpea treated by growth promoting substance had much bigger and less vacuoles than those in treated by water.The number of bacteroid in per cell and the content of leghaemoglobin of cowpea nodule treated by growth promoting substance was also remarkably increased.Meanwhile,the common peribacteroid membranes were appeared in a high frequency than those treated by water.The results showed that cowpea treated by growth promoting substance had higher production of leghaemoglobin,different microstructure and bacterioid ultrastructure of cowpea nodule which led to a higher nitrogenase activity of peanut nodule.