Studies on silk fibroin of the silkworm Bombyx mori |
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Authors: | S Sridhara J C Prudhomme J Daillie |
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Institution: | Section de Biologie Générale et Appliquée, Université Claude Bernard, 69621 Villeurbanne, France |
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Abstract: | A procedure has been developed to obtain native fibroin in a pure state from the reservoir part of the silk gland. The purified protein has a sedimentation coefficient of 10 S as determined on sucrose density gradients and the amino acid composition is similar to that reported for fibroin from the cocoons. The effects of various solvents has been studied; lithium thiocyanate was found to be the solvent of choice. By in vivo labeling of fibroin with 3H]glycine and 14C]alanine it was demonstrated that fibroin synthesized in the posterior part of the gland and that stored in the reservoir part are identical. |
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