Import of Modified Dl Protein and its Assembly into Photosystem II by Isolated Chloroplasts |
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Authors: | Wu Guo-Jiang; Watanabe Akira |
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Institution: | Department of Biological Sciences, Graduate School of Science, University of Tokyo Hongo, Bunkyo-ku, Tokyo, 113 Japan |
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Abstract: | A portion of the rbcS gene that encoded the transit peptideand 20 amino acid residues of the N-tenninal region of the smallsubunit of ribulosebisphosphate carb-oxylase/oxygenase was fusedto the 5' end of the psbA gene which encodes the Dl proteinof PSII reaction center. The chimeric gene was expressed invitro as a 42-kDa protein, which was imported into chloroplastsisolated from pea leaves. The imported protein was processedsuch that the transit peptide was lost in the stroma, the resultantprotein was translocated into thylakoid membranes, and the C-ter-minalpeptide was then removed to yield a mature protein with an N-terminalextension originated from the small sub-unit. The mature proteinappeared to be assembled into the PSII core complex, resemblingthe native Dl protein in terms of protein structure and topologywithin the membrane. Our observations indicate that the structureof the precursor to the Dl protein includes information forthe proper assembly of the protein into the PSII core complex. (Received September 10, 1996; Revision received December 14, 1996. ) |
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