Dihydrokaempferol Glucoside from Cotyledons Promotes Flowering in Pharbitis nil |
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Authors: | Nakanishi Fumi; Kusumi Takenori; Inoue Yukinobu; Fujii Tadashi |
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Institution: | 1 Institute of Biological Sciences, University of Tsukuba Tsukuba, Ibaraki, 305 Japan
2 Faculty of Pharmaceutical Sciences, University of Tokushima Tokushima, 770 Japan
3 Department of Chemistry, University of Tsukuba Tsukuba, Ibaraki, 305 Japan |
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Abstract: | An extract of cotyledons of Pharbitis nil, which had been exposedto short-day conditions, was tested for flower-promoting activityin a shoot-tip assay system in vitro. The crude extract hadno flower-promoting activity, however, after partitioning ofthe crude extract with dichloromethane, the resulting aqueousfraction had flower-promoting activity. This activity was separatedinto two fractions by column chromatography on Toyopearl HW-40.One active fraction was identified as dihydrokaempferol-7-O-rß-D-glucoside(DHK-glc). This compound exhibited flower-promoting activityat the extremely low concentration of 4.4x10-9. (Received April 25, 1995; Accepted August 11, 1995) |
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