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Induction and Repair of Damage to DNA in Cucumber Cotyledons Irradiated with UV-B |
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| Authors: | Takeuchi Yuichi; Murakami Mina; Nakajima Nobuyoshi; Kondo Noriaki; Nikaido Osamu |
| Institution: | 1Department of Bioscience and Technology, School of Engineering, Hokkaido Tokai University Sapporo, 005 Japan 2Course of Environmental and Biological Sciences, Graduate School of Science and Engineering, Hokkaido Tokai University Sapporo, 005 Japan 3Biotechnology Research Team, National Institute for Environmental Studies Tsukuba, 305 Japan 4Division of Radiation Biology, Faculty of Pharmaceutical Sciences, Kanazawa University Kanazawa, 920 Japan |
| Abstract: | Photoinduced lesions in DNA, namely, cyclobutane pyrimidinedimers (CPDs) and pyrimidine-(6-4)-pyrimidone photoproducts(6-4)photoproducts], in cucumber cotyledons that had been irradiatedwith naturally occurring levels of UV-B (290–320 nm) werequantitated by enzyme-linked immunosorbent assays with monoclonalantibodies specific to each type of photolesion. Induction ofthese photolesions was dependent on temperature and their extentwas reduced by simultaneous irradiation with white light. Thedark repair of both types of photolesion was undetectable. Light-dependentremoval of (6-4)photoproducts was very slow, with 50% removalin 4 h. By contrast, 50% of initial CPDs were removed within15 min. Both photorepair processes were dependent on the intensityof white light and were sensitive to temperature. These resultsindicate that high photolyase activity is present in cucumbercotyledons and that repair activities in cucumber cotyledonsare different from those reported in Arabidopsis, in which (6-4)photoproductsare photorepaired more rapidly than CPDs. (Received October 13, 1995; Accepted December 28, 1995) |
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