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Structural Studies of the Vacuolar H+-Pyrophosphatase: Sequence Analysis and Identification of the Residues Modified by Fluorescent Cyclohexylcarbodiimide and Maleimide |
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| Authors: | Maruyama Chio; Tanaka Yoshiyuki; Takeyasu Kunio; Yoshida Masasuke; Sato Masa H |
| Institution: | 1 Department of Natural Environmental Sciences, Faculty of Integrated Human Studies, Kyoto University Yoshidanihonmatsu-cho, Sakyo-ku Kyoto, 606-8501 Japan 2 Research Laboratory for Resources Utilization, Tokyo Institute of Technology 4259 Nagatsuta, Midori-ku, Yokohama, 226-8503 Japan 3 National Institute of Agrobiological Resources 2-1-2, Kannondai, Tsukuba, Ibaragi, 305-8602 Japan |
| Abstract: | We determined the amino acid residues of the H+-translocatinginorganic pyrophosphatase (H+-PPase) of pumpkin which are covalentlylabeled by two fluorescent labeling reagents; N-cyclohexyl-.N'-4-(dimethylamino)- |
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