Effects of some SH-inhibitors and EDTA on flowering in Lemna perpusilla 6746 |
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Authors: | Takimoto Atsushi; Tanaka Osamu |
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Institution: | Laboratory of Applied Botany, Faculty of Agriculture, Kyoto University Kyoto, Japan |
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Abstract: | Lemna perpusilla 6746, a short-day duckweed, flowered undercontinuous illumination on M-sucrose medium containing CuSO4,AgNO3 and HgCl2, which are SH-inhibitors. The optimum concentrationsof CuSO4, AgNO3 and HgCl2 were 5, 1 and 20 µM, respectively.Other metal ions tested were ineffective, but at least two otherSHinhibitors, potassium ferricyanide and iodoacetamide, alsoinduced long-day flowering at the concentrations of 0.1-1 µM. Adding 50 µM EDTA to the medium prevented the effect ofcupric ion, but not that of other SH-inhibitors. EDTA at 200µM induced some long-day flowering when added to a mediumwith no SH-inhibitors. It also permitted some flowering whenadded together with cupric ion, and accelerated flowering inthe presence of the other SHinhibitors listed above. EDTA andSH-inhibitor effects appeared to be additive. (Received May 25, 1973; ) |
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