Dimer Formation of a Cell-Cell Adhesion Protein, gp64 of the Cellular Slime Mold, Polysphondylium pallidum |
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Authors: | Ochiai Hiroshi; Hata Katsusuke; Saito Tamao; Funamoto Satoru; Nakata Nobuyuki |
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Institution: | Division of Biological Sciences, Graduate School of Science, Hokkaido University Sapporo, 060 Japan |
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Abstract: | The cellular slime mold, Polysphondylium pallidum, has two EDTA-resistanttypes of cell-cell adhesion. The major component of them hasbeen identified as a glycoprotein with a molecular mass of 64kDa on SDS-PAGE (referred to as gp64). We found that a substantialamount of the gp64 run as dimer, when gp64 was dissolved inSDS-sample buffer without 2-mercaptoethanol and then subjectedto electrophoresis. The occurrence of a homophilic dimer wasdemonstrated by analyzing the dimer-like band on a gel for itsamino acid sequence and amino acid composition. The dimer-likeband also was analyzed by three sorts of monoclonal antibodies,two of which recognize respectively a conforniational epitopeand a denatured epitope of the protein moiety of gp64. The dataindicate that the native conformation of gp64 is necessary fordimer formation.
2Present address: Institute of Immunological Science, HokkaidoUniversity, Sapporo, 060 Japan |
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