Analysis of green pit viper (Trimeresurus alborabris) venom protein by LC/MS-MS |
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| Authors: | Soogarun Suphan Sangvanich Polkit Chowbumroongkait Montri Jiemsup Surasak Wiwanikit Viroj Pradniwat Paweena Palasuwan Attakorn Pawinwongchai Jaturawat Chanprasert Supanthitra Moungkote Tusrin |
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| Institution: | Clinical Microscopy Research Unit, Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand. |
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| Abstract: | Green pit viper venom has major effect on the hematological system having a thrombin-like effect. Thus, this study is designed to analyze the composition of Trimeresurus albolabris venom by performing gel filtration and LC/MS-MS. The purified protein was then digested by trypsin, and the tryptic fragments were analyzed by iontrap spectrophotometry. This study found four types of proteins, namely jerdonitin, stejaggregin-A beta chain-1, stejnobin, and stejnihagin-A, as the components of T. albolabris venom. All of these toxins played a greater or lesser role in clot formation or otherwise contributed to cross-reactions in antivenom production. |
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