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| 单分子PCR产物错误率分析 | |
| 引用本文： | 王国华,吕军鸿,雷晓玲,李海阔,李民乾,陈润生,方海平,胡钧.单分子PCR产物错误率分析[J].生物化学与生物物理进展,2004,31(2):159-162. |
| 作者姓名： | 王国华 吕军鸿 雷晓玲 李海阔 李民乾 陈润生 方海平 胡钧 |
| 作者单位： | 1. 中国科学院上海应用物理研究所,上海,201800 2. 上海交通大学Bio-X生命科学研究中心,上海,200030 3. 中国科学院生物物理研究所,北京,100101 4. 中国科学院上海应用物理研究所,上海,201800;上海交通大学Bio-X生命科学研究中心,上海,200030 |
| 基金项目： | 国家自然科学基金重点项目(10335070). |
| 摘 要： | 碱基错配致使PCR扩增产物中存在突变序列.大量模板PCR扩增时突变序列所占的比例较低，对随后进行的PCR产物分析影响不大，但当对微量甚至单个模板DNA扩增时，情况则完全不同.对单分子PCR产物的错误率进行了理论分析，结果表明：根据实验目的和条件，选择忠实性不同的聚合酶是十分关键的. |
| 关 键 词： | 单分子PCR，错误率，忠实性，模板数 |
| 收稿时间： | 2003/6/30 0:00:00 |
| 修稿时间： | 2003/9/28 0:00:00 |
Error Rate in Single Molecule PCR |
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| Institution: | Nanobiology and Nanomedicine Laboratory, Shanghai Institute of Applied Physics, The Chinese Academy of Sciences, Shanghai 201800, China;Nanobiology and Nanomedicine Laboratory, Shanghai Institute of Applied Physics, The Chinese Academy of Sciences, Shanghai 201800, China;Nanobiology and Nanomedicine Laboratory, Shanghai Institute of Applied Physics, The Chinese Academy of Sciences, Shanghai 201800, China;Bio-X Life Sciences Research Center, Shanghai JiaoTong University, Shanghai 200030, China;Nanobiology and Nanomedicine Laboratory, Shanghai Institute of Applied Physics, The Chinese Academy of Sciences, Shanghai 201800, China;Molecular Biology Research Center, Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China;Nanobiology and Nanomedicine Laboratory, Shanghai Institute of Applied Physics, The Chinese Academy of Sciences, Shanghai 201800, China;Nanobiology and Nanomedicine Laboratory, Shanghai Institute of Applied Physics, The Chinese Academy of Sciences, Shanghai 201800, China;Bio-X Life Sciences Research Center, Shanghai JiaoTong University, Shanghai 200030, China |
| Abstract: | Mismatches during amplification result in some mutations in the PCR products. The simulation indicates that the rate of mutation is quite low and has no influence on the consequent sequencing analysis when template DNA molecules are abundant, but it is not the case when the PCR amplification is performed with single or few molecules as templates. The error rates of single molecule PCR under different conditions showed that it is essential to select different polymerases according to different experimental purposes. |
| Keywords: | single molecule PCR error rate fidelity the number of template molecule |
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