| 水稻 Ca2+/H+ 反向转运体 OsCAX3 的功能分析和亚细胞定位研究 |
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| 引用本文： | 祁碧菽,李春光,陈叶苗,陆平利,郝福顺,沈国明,陈 珈,王学臣.水稻 Ca2+/H+ 反向转运体 OsCAX3 的功能分析和亚细胞定位研究[J].生物化学与生物物理进展,2005,32(9):876-882. |
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| 作者姓名： | 祁碧菽 李春光 陈叶苗 陆平利 郝福顺 沈国明 陈 珈 王学臣 |
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| 作者单位： | 中国农业大学生物学院植物学系,国家植物生理生化重点实验实,北京,100094 |
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| 基金项目： | 国家重点基础研究发展规划（973）（2006 CB100100和2003CB114300），国家自然科学基金资助项目（30370129和30421002）. |
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| 摘 要： | Ca2+/H+ 反向转运体作为一类 Ca2+外向转运器，在植物的营养和信号转导中起着非常重要的作用 . 克隆了水稻 Ca2+/H+ 反向转运体基因 OsCAX3 ，序列分析表明 OsCAX3 具有 11 个跨膜区，其中在第 6 和第 7 个跨膜区之间有一个 17 个氨基酸组成的酸性基序 (acid motif) ，功能互补实验证明 OsCAX3 具有转运 Ca2+ 的功能，并且其 N 端 26 个氨基酸序列对转运 Ca2+ 具有一定的抑制作用 . RT-PCR 分析表明 OsCAX3 的表达受到外源 Ca2+ 的诱导 . 利用 PSORT prediction 进行亚细胞定位分析，和利用 OsCAX3-GFP 融合蛋白瞬时表达分析证明， OsCAX3 定位于细胞质膜 . 以上结果表明， OsCAX3 是一种定位于细胞质膜上的 Ca2+/H+ 反向转运体 .
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| 关 键 词： | 水稻， Ca2+/H+ 反向转运体， RT-PCR ，原生质体，瞬时表达 |
| 收稿时间： | 04 22 2005 12:00AM |
| 修稿时间： | 2005-04-222005-06-29 |
Functional Analysis of Rice Ca2+/H+ Antiporter OsCAX3 in Yeast and Its Subcellular Localization in Plant |
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| QI Bi-Shu,LI Chun-Guang,CHEN Ye-Miao,LU Ping-Li,HAO Fu-Shun,SHEN Guo-Ming,CHEN Jia and WANG Xue-Chen.Functional Analysis of Rice Ca2+/H+ Antiporter OsCAX3 in Yeast and Its Subcellular Localization in Plant[J].Progress In Biochemistry and Biophysics,2005,32(9):876-882. |
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| Authors: | QI Bi-Shu LI Chun-Guang CHEN Ye-Miao LU Ping-Li HAO Fu-Shun SHEN Guo-Ming CHEN Jia and WANG Xue-Chen |
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| Institution: | College of Biological Science, China Agricultural University, State Key Laboratory of Plant Physiology and Biochemistry, Beijing 100094, China;College of Biological Science, China Agricultural University, State Key Laboratory of Plant Physiology and Biochemistry, Beijing 100094, China;College of Biological Science, China Agricultural University, State Key Laboratory of Plant Physiology and Biochemistry, Beijing 100094, China;College of Biological Science, China Agricultural University, State Key Laboratory of Plant Physiology and Biochemistry, Beijing 100094, China;College of Biological Science, China Agricultural University, State Key Laboratory of Plant Physiology and Biochemistry, Beijing 100094, China;College of Biological Science, China Agricultural University, State Key Laboratory of Plant Physiology and Biochemistry, Beijing 100094, China;College of Biological Science, China Agricultural University, State Key Laboratory of Plant Physiology and Biochemistry, Beijing 100094, China;College of Biological Science, China Agricultural University, State Key Laboratory of Plant Physiology and Biochemistry, Beijing 100094, China |
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| Abstract: | Ca2+/H+ antiporters play important roles in plant nutrition and signal transduction. A novel Ca2+/H+ antiporters gene OsCAX3 was identified from rice. Sequence analysis revealed that OsCAX3 has 11 transmembrane domains, and an acid motif consisting of 17 amino acids between sixth and seventh transmembrane domains. When expressed in yeast, OsCAX3 complemented the mutant growing deficiency at high Ca2+ concentrations, and its N-terminal(1~26 amino acid) partially suppressed its ability to transport Ca2+. RT-PCR analysis showed that OsCAX3 mRNA was induced by exogenous calcium. OsCAX3 was predicted to localize in plant plasma membrane by PSORT prediction, and this was further confirmed by transiently expressing OsCAX3-GFP fusion protein in mesophyll cell protoplast of Arabidopsis. All these findings strongly suggested that OsCAX3 is a Ca2+/H+ antiporter located in plasma membrane. |
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| Keywords: | rice Ca2+/H+ antiporter RT-PCR protoplast transient expression |
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