| 钙指示剂的发展及其研究现状 |
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| 引用本文： | 李佳,王友军,张晓嫣.钙指示剂的发展及其研究现状[J].生物化学与生物物理进展,2021,48(7):788-806. |
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| 作者姓名： | 李佳 王友军 张晓嫣 |
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| 作者单位： | 2）北京师范大学生命科学学院生物系，抗性基因资源与分子发育北京市重点实验室，北京 100875,2）北京师范大学生命科学学院生物系，抗性基因资源与分子发育北京市重点实验室，北京 100875,1）北京师范大学生命科学学院生物系，细胞增殖及调控生物学教育部重点实验室，北京 100875 |
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| 摘 要： | 钙指示剂常被用于细胞及细胞器钙信号的检测,是钙信号转导研究中必不可少的工具.目前的钙指示剂分两大类,包括化学钙指示剂,如Fura-2、Indo-1、Fluo-4等,和基因编码的钙指示蛋白如D1ER、GCaMP、CEPIA1er等.随着技术的发展及研究需求的不断提升,各版本的钙指示剂也在不断更新.本文对已有的钙指示剂进行了系统地梳理,详细介绍了目前应用最为广泛的钙指示剂,总结了现有钙指示剂的优缺点,并对可优化提升的部分进行了展望,旨在为钙指示剂研究的进一步发展提供一些思路.
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| 关 键 词： | 钙指示剂 钙信号 钙成像 |
| 收稿时间： | 2020/10/12 0:00:00 |
| 修稿时间： | 2021/1/2 0:00:00 |
Research Progress on Calcium Indicators |
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| LI Ji,WANG You-Jun and ZHANG Xiao-Yan.Research Progress on Calcium Indicators[J].Progress In Biochemistry and Biophysics,2021,48(7):788-806. |
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| Authors: | LI Ji WANG You-Jun and ZHANG Xiao-Yan |
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| Institution: | 2)Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China,2)Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China,1)Ministry of Education Key Laboratory of Cell Proliferation and Regulation Biology, College of Life Sciences, Beijing Noamal University, Beijing 100875, China |
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| Abstract: | Calcium indicators are often used to monitor calcium signaling in cells and organelles, which include chemical calcium indicators and genetically encoded calcium indicators. With the development of technology and the continuous increase in research requirements, various versions of calcium indicators are constantly updated. The review systematically sorts out the existing calcium indicators, and introduces in detail the most widely used calcium indicators. Here, the advantages and disadvantages of existing calcium indicators are summarized, and the parts that can be optimized and improved are discussed. The aim is to provide some ideas for the further development of calcium indicator research. |
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| Keywords: | calcium indicators calcium signaling calcium imaging |
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