| 人FKBP52的基因克隆、表达及活性研究 |
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| 引用本文： | 裴武红,贺永怀,陈兴,李松,沈倍奋.人FKBP52的基因克隆、表达及活性研究[J].生物化学与生物物理进展,2001,28(2):236-239. |
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| 作者姓名： | 裴武红 贺永怀 陈兴 李松 沈倍奋 |
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| 作者单位： | 1. 北京基础医学研究所，北京 100850
2. 北京毒物药物研究所，北京 100850 |
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| 基金项目： | 国家“973”创新药物基金资助项目(G1998051107). |
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| 摘 要： | 为获得具有生物学活性的hFKBP52，来筛选新型的促神经再生药物.采用半巢式、桥联PCR及亲和层析方法，从人胎脑cDNA文库中成功扩增出hFKBP52基因，在pET28a（+）中实现了高效、可溶性的融合表达，表达量约30%.重组的蛋白质经亲和纯化至电泳纯，纯化后的hFKBP52显示出肽基脯氨基顺反异构酶活性.表明原核表达的hFKBP52具有类似于其天然蛋白质的生物学活性.
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| 关 键 词： | 人FKBP52，基因克隆，原核表达，肽基脯氨基顺反异构酶 |
| 收稿时间： | 2000/4/17 0:00:00 |
| 修稿时间： | 2000年4月17日 |
The Gene Cloning, Expression and Bioactivity of the Human FKBP52 |
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| PEI Wu-Hong,HE Yong-Huai,CHEN Xing,LI Song and SHEN Bei-Fen.The Gene Cloning, Expression and Bioactivity of the Human FKBP52[J].Progress In Biochemistry and Biophysics,2001,28(2):236-239. |
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| Authors: | PEI Wu-Hong HE Yong-Huai CHEN Xing LI Song and SHEN Bei-Fen |
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| Institution: | Beijing Institute of Basic Medical Sciences, Beijing 100850, China;Beijing Institute of Basic Medical Sciences, Beijing 100850, China;Beijing Institute of Basic Medical Sciences, Beijing 100850, China;Beijing Institute of pharmacology and toxicology, Beijing 100850, China;Beijing Institute of Basic Medical Sciences, Beijing 100850, China |
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| Abstract: | To obtain active hFKBP52 protein for screening novel neurotrophic drugs. Semi-nested and overlap PCR and affinity chromatography were used. hFKBP52 gene was cloned successfully from human fetal brain cDNA library, and then highly expressed (about 30%) as fusion protein in pET28a(+) vector system. The recombinant protein was purified as one band on SDS-PAGE. The purified hFKBP52 showed peptidyl-prolyl cis-trans isomerase (PPIase) activity, similar to the wild type. |
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| Keywords: | hFKBP52 gene clone proyoatic expression peptidyl-prolyl cis-trans isomerase |
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