细胞黏附分子互作网络的构建与分析

借助网络分析可对基因调控、蛋白质互作和信号转导等细胞活动进行全局和局部性质分析.以细胞黏附的蛋白质相互作用为对象,通过数据挖掘和可视化软件构建了整合蛋白介导的黏附分子互作网络,该分子互作网络由156种蛋白质通过690种相互作用相连,其平均节点度为8.66、平均聚集系数为0.24,平均路径长度为2.6.黏附分子互作网络中包含数个功能模块,这些模块涉及网络内部多种分子相互作用的启动与停止,并进一步影响细胞的黏附、迁移和骨架组织.对黏附分子网络进行模体筛选和比较,发现一些数量相对较少、以三元复合物为主要结构的关键模体,同时对各网络模块和模体对细胞黏附的调控作用进行了探讨.

Construction and analysis for molecular interaction network of cell adhesion

Complex network has been used to analyze the global and local properties of cell processes such as gene regulation, protein-protein interaction and signal transduction. A molecular network of integrin-mediated cell adhesions is constructed by data-mining and presented by a visualizing software. The network consists of 156 components linked by 690 interactions, with an average node degree of 8.66, an average cluster coefficient of 0.24 and an average path length of 2.6. Several functional modules are involved in switching on or off many of the molecular interactions within the network, consequently affecting cell adhesion, migration and cytoskeletal organization. Screening and examination of the adhesome network motifs reveals a relatively small number of key motifs, dominated by 3-nodes (three-component complexes). The role of the different network modules and modifs in regulating cell adhesions is also discussed.