| 某些肿瘤组织中血管内皮生长因子基因的表达 |
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| 引用本文： | 修波,于昕,李惠芳,周爱儒.某些肿瘤组织中血管内皮生长因子基因的表达[J].生物化学与生物物理进展,1994,21(5):426-429. |
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| 作者姓名： | 修波 于昕 李惠芳 周爱儒 |
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| 作者单位： | 青岛医学院生物化学教研室;北京医科大学生物化学教研室;北京医科大学生物化学教研室;北京医科大学生物化学教研室 |
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| 摘 要： | 血管内皮生长因子(vascular endothelial growth factors,VEGF)是新发现的生长因子,特异作用于血管内皮细胞,促进其增殖及新生血管的生成.已确认,它和实体肿瘤的生长有着十分密切的关系.文章报道,利用力子杂交技术分析了胃癌、肾癌、结肠癌和膀胱癌及其癌旁相应组织中VEGF mRNA的表达,结果发现,癌组织较其癌旁组织中vEGF mRNA的表达增高.SGC-7901细胞加TPA 4h后则明显促进VEGF mRNA的表达.转染含人反义N-ras1 DNA片段重组质粒的人膀胱癌BIU-87细胞系可抑制VEGFmRNA表达.
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| 关 键 词： | 血管内皮生长因子 点杂交 肿瘤 SGC－7901细胞系 mRNA表达 |
| 收稿时间： | 1993/7/20 0:00:00 |
| 修稿时间： | 1993/9/11 0:00:00 |
Vascular Endothelial Growth Factor Gene Expression in Some Tumors |
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| Xiu Bo,Yu Xin,Li Huifang and Zhou Airu.Vascular Endothelial Growth Factor Gene Expression in Some Tumors[J].Progress In Biochemistry and Biophysics,1994,21(5):426-429. |
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| Authors: | Xiu Bo Yu Xin Li Huifang and Zhou Airu |
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| Institution: | Deparrment of Biochemistry, Beijing Medical University;Deparrment of Biochemistry, Beijing Medical University;Deparrment of Biochemistry, Beijing Medical University;Deparrment of Biochemistry, Beijing Medical University |
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| Abstract: | Vascular endothelial growth factor (VEGF) is a specific mitogen on endothelial cells and promotes angiogenesis. It has been identified that VEGF is very closely linked to the growth of solid tumors. With Dot blot technique, expression of the VEGF mRNA in cancers of stomach, kidney, bladder and colon are investigated. The results showed that the expression of the VEGF mRNA is higher in tumors than in peritumors. There is significant expression of VEGF mRNA in SGC-7901 cells after adding TPA 4h. Human bladder carcinoma cell line BIU-87 transfected with antisense N-ras exon 1 DNA fragment inhibit VEGF mRNA expression. |
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| Keywords: | vascular endothelial growth factor Dot blot tumor SGC-7901 cell line mRNA expression |
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