| 体细胞克隆山羊微卫星DNA分析 |
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| 引用本文： | 郭泽坤,郭继彤,安志兴,张涌,柴玉波,陈南春,陈苏民.体细胞克隆山羊微卫星DNA分析[J].生物化学与生物物理进展,2002,29(4):655-658. |
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| 作者姓名： | 郭泽坤 郭继彤 安志兴 张涌 柴玉波 陈南春 陈苏民 |
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| 作者单位： | 1. 西北农林科技大学生物工程研究所,杨陵,712100
2. 第四军医大学全军分子生物学重点实验室,西安,710021 |
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| 基金项目： | 国家自然科学基金资助项目（39830280）. |
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| 摘 要： | 用10对山羊微卫星DNA多态性引物对2只体细胞克隆济宁青山羊、青山羊供体细胞、受体奶山羊母羊以及具有亲缘关系的3只对照济宁青山羊进行微卫星DNA分析.结果表明有5对山羊微卫星DNA多态性引物，即SR-CRSP1, SR-CRSP5, SR-CRSP6, SR-CRSP7和SR-CRSP24，扩增产物有明显的多态性.扩增产物经过6%变性聚丙烯酰胺凝胶电泳后银染，结果2只体细胞克隆山羊的微卫星DNA指纹与供体细胞完全相同，而且不同于其受体母亲也不同于其他所有同品种不同个体的对照青山羊.证明体细胞克隆山羊基因组来源于供体细胞.
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| 关 键 词： | 体细胞，克隆山羊，微卫星DNA，PCR，聚丙烯酰胺，电泳，银染 |
| 收稿时间： | 2001/12/28 0:00:00 |
| 修稿时间： | 2001年12月28 |
Microsatellite DNA Analysis of Somatic Cloned Goats |
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| GUO Ze-Kun,GUO Ji-Tong,AN Zhi-Xing,ZHANG Yong,CHAI Yu-Bo,CHEN Nan-Chun and CHEN Su-Min.Microsatellite DNA Analysis of Somatic Cloned Goats[J].Progress In Biochemistry and Biophysics,2002,29(4):655-658. |
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| Authors: | GUO Ze-Kun GUO Ji-Tong AN Zhi-Xing ZHANG Yong CHAI Yu-Bo CHEN Nan-Chun and CHEN Su-Min |
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| Institution: | Institute of Bioengineering, Northwestern Science-technology University of Agriculture & Forestry, Yangling 712100, China;Institute of Bioengineering, Northwestern Science-technology University of Agriculture & Forestry, Yangling 712100, China;Institute of Bioengineering, Northwestern Science-technology University of Agriculture & Forestry, Yangling 712100, China;Institute of Bioengineering, Northwestern Science-technology University of Agriculture & Forestry, Yangling 712100, China;PLA Key Laboratory of Molecular Biology, The Fourth Military Medical University, Xi'an 710021, China;PLA Key Laboratory of Molecular Biology, The Fourth Military Medical University, Xi'an 710021, China;PLA Key Laboratory of Molecular Biology, The Fourth Military Medical University, Xi'an 710021, China |
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| Abstract: | Microsatellite DNA analysis was performed using ten pairs of goat microsatallite polymorphic DNA primers on two somatic cloned Jining grey goats, donor grey goat, recipient goats and three related control Jining grey goats. The result suggests that the amplification products are remarkably polymorphic in five pairs of goat microsatallite polymorphic DNA primers: SR-CRSP1, SR-CRSP5, SR-CRSP6, SR-CRSP7 and SR-CRSP24.The amplification products were silver stained after running on the 6% SDS-PAGE. The microsatellite DNA fingerprints of the two somatic cloned goats are the same as the donor, but are different from the recipient goat and all the control grey goats. It proves that the genomes of the two somatic cloned goats come from the donor cells. |
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| Keywords: | somatic cell cloned goat microsatellite DNA PCR polyacrolymide electrophoresis silver staining |
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