转移缓冲液对蛋白质印迹的影响

利用三种转移缓冲液，分别以半干和湿转法，检测癌基因产物Bcl-2, 并获得不同强度的印迹结果.不含SDS的转移缓冲液转移效果明显优于含SDS的转移缓冲液，且SDS含量越高印迹带越弱；此外，半干转移与湿转相比，可大大缩短转移时间，但转移效果不及湿转理想且稳定性较差.

Comparison of Three Transfer Buffer in the Protein Blotting with Semidry and Tank Electric Transfer

In western blotting, the electrophoretic transfer effect of the proteins can be affected by sodium dodecyl sulfonate (SDS) in the transfer buffer. Using three transfer buffer with or without SDS in both semidry and tank transfer systems, the Bcl-2 protein levels were tested and the spectrum of the protein blots were compared. The addition of SDS in buffer reduced the binding of Bcl-2 protein with PVDF membrane, either in semidry or tank electric transfer. The transfer efficiency of both semidry and tank systems were also compared. The semidry electric transfer markedly minimized the transfer times, but the transfer result were a little poorer than the tank transfer system.