| 一种改进的蛋白质超薄凝胶电泳方法 |
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| 引用本文： | 郭泽坤,张涌.一种改进的蛋白质超薄凝胶电泳方法[J].生物化学与生物物理进展,2000,27(1):98-101. |
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| 作者姓名： | 郭泽坤 张涌 |
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| 作者单位： | 西北农业大学发育生物学实验室,陕西杨凌,712100 |
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| 基金项目： | 国家自然科学基金资助项目（39570526）. |
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| 摘 要： | 介绍了一种将聚丙烯酰胺凝胶固定在电泳夹板上的蛋白质电泳方法.通过此方法蛋白质电泳可以在0.4 mm厚的聚丙烯酰胺凝胶上进行.实验证明，经此方法处理的玻板结合凝胶非常牢固，在电泳后的所有处理步骤中都不会发生凝胶脱落现象.
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| 关 键 词： | 电泳，聚丙烯酰胺，蛋白质，超薄凝胶电泳 |
| 收稿时间： | 1998/11/13 0:00:00 |
| 修稿时间： | 1999/3/29 0:00:00 |
An Improved Method for Ultrathin-Gel Electrophoresis of Protein |
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| GUO Ze-Kun and ZHANG Yong.An Improved Method for Ultrathin-Gel Electrophoresis of Protein[J].Progress In Biochemistry and Biophysics,2000,27(1):98-101. |
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| Authors: | GUO Ze-Kun and ZHANG Yong |
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| Institution: | Laboratory of Developmental Biology, Northwestern Agricultural University, Yangling, Shaanxi 712100, China;Laboratory of Developmental Biology, Northwestern Agricultural University, Yangling, Shaanxi 712100, China |
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| Abstract: | An improved method for ultrathin-gel electrophoresis of protein was developed, which bind polyacrylamide gel to glass. By this method electrophoresis of protein can be run on 0.4 mm thick gel. It was demonstrated that glass treated by this method could bind gel tightly throughout all the procedures after electrophoresis. |
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| Keywords: | electrophoresis polyacrylamide protein ultrathin-gel electrophoresis |
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