| 单管直扩38重祖先信息InDels体系的构建及其在微流控芯片系统的应用 |
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| 引用本文： | 韩俊萍,赵蕾,王庆国,江丽,谢何鑫,庄斌,赵丽健,李唐松,吴瑾,李彩霞.单管直扩38重祖先信息InDels体系的构建及其在微流控芯片系统的应用[J].生物化学与生物物理进展,2020,47(9):969-982. |
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| 作者姓名： | 韩俊萍 赵蕾 王庆国 江丽 谢何鑫 庄斌 赵丽健 李唐松 吴瑾 李彩霞 |
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| 作者单位： | 公安部物证鉴定中心 法医遗传学公安部重点实验室,公安部物证鉴定中心 法医遗传学公安部重点实验室,山东第一医科大学;山东第一医科大学,公安部物证鉴定中心 法医遗传学公安部重点实验室,公安部物证鉴定中心 法医遗传学公安部重点实验室,北京博奥晶典生物技术有限公司,北京博奥晶典生物技术有限公司,北京市公安局朝阳分局,北京市公安局朝阳分局,公安部物证鉴定中心 法医遗传学公安部重点实验室 |
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| 摘 要： | 本文基于插入-缺失多态性(insertion-deletion polymorphism,InDel)遗传标记,从相关文献和dbSNP库中筛选出38个在东亚、欧洲、非洲人群中具有等位基因频率差异的祖先来源InDel位点,同时整合Amelogenin位点和Y染色体STR(DYS439)位点,以辅助未知样本的性别鉴定,采用PCR-CE技术构建了可以区分三大洲际人群的单管直接扩增复合检测体系,该体系可与微流控芯片系统相结合,1.7 h内完成DNA样本自动化扩增和电泳分型,利用公共数据库1000Genomes中16个人群1 607名个体的分型数据对筛选的38个InDel位点进行初步评估;同时对构建的38-plex InDels体系的灵敏度、准确性、直接扩增能力进行验证评估;检测5种不同人群的779份样本和215份唾液卡、血卡的直接扩增样本,采用聚类分析和主成分分析方法,评价体系的推断准确性.结果表明该38-plex InDels复合检测体系分型准确,灵敏度达157 pg,能够对唾液卡和血卡直接扩增,可以区分三大洲际人群及其混合人群,能够准确推断待测样本的族群来源、估算祖先成分,且通过集成细胞裂解步骤将有望2h实现"样本进-结果出"式快速自动化InDel分型.
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| 关 键 词： | 法医物证学 插入-缺失多态性 祖先信息位点 微流控芯片 |
| 收稿时间： | 2020/1/14 0:00:00 |
| 修稿时间： | 2020/5/11 0:00:00 |
A single-tube direct amplification 38-plex ancestry informative InDels assay and its integration with a microfluidic system* |
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| HAN Jun-Ping,ZHAO Lei,WANG Qing-Guo,JIANG Li,XIE He-Xin,ZHUANG Bin,ZHAO Li-Jian,LI Tang-Song,WU Jin and LI Cai-Xia.A single-tube direct amplification 38-plex ancestry informative InDels assay and its integration with a microfluidic system*[J].Progress In Biochemistry and Biophysics,2020,47(9):969-982. |
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| Authors: | HAN Jun-Ping ZHAO Lei WANG Qing-Guo JIANG Li XIE He-Xin ZHUANG Bin ZHAO Li-Jian LI Tang-Song WU Jin and LI Cai-Xia |
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| Institution: | Key Laboratory of Forensic Genetics,Beijing Engineering Research Center of Crime Scene Evidence Examination,National Engineering Laboratory for Forensic Science,Institute of Forensic Science,Key Laboratory of Forensic Genetics,Beijing Engineering Research Center of Crime Scene Evidence Examination,National Engineering Laboratory for Forensic Science,Institute of Forensic Science,Shandong First Medical University,Key Laboratory of Forensic Genetics,Beijing Engineering Research Center of Crime Scene Evidence Examination,National Engineering Laboratory for Forensic Science,Institute of Forensic Science,Key Laboratory of Forensic Genetics,Beijing Engineering Research Center of Crime Scene Evidence Examination,National Engineering Laboratory for Forensic Science,Institute of Forensic Science,Beijing CapitalBio Technology Ltd Co,Beijing,Beijing CapitalBio Technology Ltd Co,Beijing,Technology Department of Chaoyang Sub-bureau,Beijing Public Security Bureau,Beijing,,China,Technology Department of Chaoyang Sub-bureau,Beijing Public Security Bureau,Beijing,,China,Key Laboratory of Forensic Genetics,Beijing Engineering Research Center of Crime Scene Evidence Examination,National Engineering Laboratory for Forensic Science,Institute of Forensic Science |
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| Abstract: | |
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| Keywords: | forensic biological evidence Insertion-Deletion polymorphisms ancestry informative markers microfluidic chip |
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