| 人胃癌细胞一种高表达基因的克隆及序列测定 |
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| 引用本文： | 赵锦荣,阎小君,韩锋产,崔大祥,侯瑜,严泉剑,苏成芝.人胃癌细胞一种高表达基因的克隆及序列测定[J].生物化学与生物物理进展,2001,28(1):99-102. |
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| 作者姓名： | 赵锦荣 阎小君 韩锋产 崔大祥 侯瑜 严泉剑 苏成芝 |
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| 作者单位： | 第四军医大学基因诊断研究所, |
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| 摘 要： | 采用DDRT-PCR法，以人胃上皮细胞GES-1为对照，从人胃癌细胞SGC-7901中克隆高表达基因，并对所克隆的基因进行斑点杂交分析, 序列测定，序列相似性分析及开放读框分析.斑点杂交分析结果表明所获克隆YA61为人胃癌细胞SGC-7901中高表达基因.序列相似性分析结果表明该基因序列为一新基因序列.GenBank收录号为AF220415.开放读框分析结果表明该基因序列有一完全开放读框.
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| 关 键 词： | 胃上皮细胞，胃癌，差异表达基因 |
| 收稿时间： | 2000/2/22 0:00:00 |
| 修稿时间： | 2000/3/31 0:00:00 |
Overexpressed Gene YA61 Cloned from Human Gastric Carcinoma Cell SGC-7901 and Its Sequencing |
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| ZHAO Jin-Rong,YAN Xiao-Jun,HAN Feng-Chan,CUI Da-Xiang,HOU Yu,YAN Quan-Jian and SU Cheng-Zhi.Overexpressed Gene YA61 Cloned from Human Gastric Carcinoma Cell SGC-7901 and Its Sequencing[J].Progress In Biochemistry and Biophysics,2001,28(1):99-102. |
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| Authors: | ZHAO Jin-Rong YAN Xiao-Jun HAN Feng-Chan CUI Da-Xiang HOU Yu YAN Quan-Jian and SU Cheng-Zhi |
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| Institution: | Institute of Genetic Diagnosis, the Fourth Military Medical University, Xi'an 710032, China;Institute of Genetic Diagnosis, the Fourth Military Medical University, Xi'an 710032, China;Institute of Genetic Diagnosis, the Fourth Military Medical University, Xi'an 710032, China;Institute of Genetic Diagnosis, the Fourth Military Medical University, Xi'an 710032, China;Institute of Genetic Diagnosis, the Fourth Military Medical University, Xi'an 710032, China;Institute of Genetic Diagnosis, the Fourth Military Medical University, Xi'an 710032, China;Institute of Genetic Diagnosis, the Fourth Military Medical University, Xi'an 710032, China |
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| Abstract: | To clone overexpressed gene from human gastric carcinoma cell SGC-7901, DDRT-PCR technique is used with human gastric epithelial cell GES-1 as control. After cloned into pGEM?-T vector, YA61,one of the overexpressed genes, was analyzed by dot blot and was sequenced then. The sequence gotten was then compared to GenBank data and analyzed by NCBI ORF Finder. Dot blot results showed that the gene YA61 was overexpressed in human gastric carcinoma cell SGC-7901. NCBI's sequence similarity search indicated that the gene YA61 was a new gene sequence. Open reading frame analysis demonstrated that the gene YA61 had one complete open reading frame. In conclusion, the gene YA61 was a new gene sequence that was overexpressed in human gastric carcinoma cell SGC-7901. |
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| Keywords: | gastric epithelial cell gastric carcinoma differentially expressed gene |
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