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| 微流控芯片检测肺癌患者血浆中p16基因异常甲基化在临床应用的评价 | |
| 引用本文： | 汪洋,邹丽娟,许冠东,宫琳琳,周小棉,邵淑娟.微流控芯片检测肺癌患者血浆中p16基因异常甲基化在临床应用的评价[J].生物化学与生物物理进展,2004,31(12):1085-1090. |
| 作者姓名： | 汪洋 邹丽娟 许冠东 宫琳琳 周小棉 邵淑娟 |
| 作者单位： | 1. 大连医科大学组织学与胚胎学教研室,大连,116027 2. 大连医科大学附属第二医院肿瘤科,大连,116027 3. 中国医学科学院肿瘤研究所病因研究室,北京,100021 4. 中国科学院化学物理研究所,大连,116025 5. 大连医科大学组织学与胚胎学教研室,大连,116027;中国医学科学院肿瘤研究所病因研究室,北京,100021 |
| 基金项目： | 国家重点基础研究发展规划资助项目(973) (2002CB513106)和辽宁省教委科技资助项目(202203248). |
| 摘 要： | 肺癌是常见的恶性肿瘤之一，其死亡率和发病率在世界范围的肿瘤性疾病中均居高不下.p16基因启动子区异常甲基化被认为是肺癌发生中的一起早期事件.为了提高检测异常甲基化方法的灵敏度及特异性，利用微流控芯片检测p16基因的异常甲基化，通过对肺癌患者血浆标本的检测，使病人血浆中的p16基因甲基化的异常改变可能成为辅助肺癌早期诊断和高危人群筛选的分子标记物，以期建立一种崭新而可靠的早期肺癌临床诊断方法. |
| 关 键 词： | p16基因，甲基化，微流控芯片，甲基化特异性PCR |
| 收稿时间： | 2004/6/17 0:00:00 |
| 修稿时间： | 8/2/2004 12:00:00 AM |
The Evaluation in Clinical Application of Detecting Aberrant Methylation of p16 Gene by Microfluidic Chips in Lung Cancer Patients |
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| WANG Yang,ZOU Li-Juan,XU Guan-Dong,GONG Lin-Lin,ZHOU Xiao-Mian and SHAO Shu-Juan.The Evaluation in Clinical Application of Detecting Aberrant Methylation of p16 Gene by Microfluidic Chips in Lung Cancer Patients[J].Progress In Biochemistry and Biophysics,2004,31(12):1085-1090. | |
| Authors: | WANG Yang ZOU Li-Juan XU Guan-Dong GONG Lin-Lin ZHOU Xiao-Mian and SHAO Shu-Juan |
| Institution: | Department of histology and embryology, Dalian Medical University, Dalian 116027, China;Department of Oncology, The Second Afflicated Hospital, Dalian Medical University, Dalian 116027, China;Department of Chemical Etiology and Carcinogenesis, Cancer Institute, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100021, China;Department of histology and embryology, Dalian Medical University, Dalian 116027, China;Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116025, China;Department of histology and embryology, Dalian Medical University, Dalian 116027, China;Department of Chemical Etiology and Carcinogenesis, Cancer Institute, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100021, China |
| Abstract: | Lung cancer is one of the malignant tumors. The death rate and incidence are very high through the whole world. Aberrant methylation of p16 gene promoter is thought to be an early event in lung cancer development. To improve the sensitivity and specificity, the microfluidic chip method was established to detect the aberrant methylation of p16 gene. The plasma samples of lung cancer patients were detected. The p16 gene methylation can perhaps become a biomarker for early lung cancer. It may become a new and reliable method for early diagnosis of lung cancer. |
| Keywords: | p16 gene methylation microfluidic chips methylation-specific PCR |
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